UMMS Affiliation

Department of Microbiology and Physiological Systems

Publication Date

2020-11-09

Document Type

Article

Disciplines

Digestive System Diseases | Disease Modeling | Immunology and Infectious Disease | Microbiology

Abstract

Crohn's disease (CD) is a chronic immune-mediated inflammatory condition caused by the loss of mucosal tolerance toward the commensal microbiota. On average, 29.5% and 42.7% CD patients experience perianal complications at 10 and 20 y after diagnosis, respectively. Perianal CD (pCD) result in high disease burden, diminished quality of life, and elevated health-care costs. Overall pCD are predictors of poor long-term outcomes. Animal models of gut inflammation have failed to fully recapitulate the human manifestations of fistulizing CD. Here, we evaluated dogs with spontaneous canine anal furunculosis (CAF), a disease with clinical similarities to pCD, as a surrogate model for understanding the microbial contribution of human pCD pathophysiology. By comparing the gut microbiomes between dogs suffering from CAF (CAF dogs) and healthy dogs, we show CAF-dog microbiomes are either very dissimilar (dysbiotic) or similar (healthy-like), yet unique, to healthy dog's microbiomes. Compared to healthy or healthy-like CAF microbiomes, dysbiotic CAF microbiomes showed an increased abundance of Bacteroides vulgatus and Escherichia coli and a decreased abundance of Megamonas species and Prevotella copri. Our results mirror what have been reported in previous microbiome studies of patients with CD; particularly, CAF dogs exhibited two distinct microbiome composition: dysbiotic and healthy-like, with determinant bacterial taxa such as E. coli and P. copri that overlap what it has been found on their human counterpart. Thus, our results support the use of CAF dogs as a surrogate model to advance our understanding of microbial dynamics in pCD.

Keywords

canine furunculosis, dysbiosis, fistulizing Crohn’s disease, microbiome, perianal fistulas

Rights and Permissions

Copyright © 2020 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

DOI of Published Version

10.1080/19490976.2020.1785246

Source

Maldonado-Contreras A, Ferrer L, Cawley C, Crain S, Bhattarai S, Toscano J, Ward DV, Hoffman A. Dysbiosis in a canine model of human fistulizing Crohn's disease. Gut Microbes. 2020 Nov 9;12(1):1785246. doi: 10.1080/19490976.2020.1785246. Epub 2020 Jul 30. PMID: 32730134; PMCID: PMC7524328. Link to article on publisher's site

Journal/Book/Conference Title

Gut microbes

Related Resources

Link to Article in PubMed

PubMed ID

32730134

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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