Mass Spectrometry Facility; Department of sBiochemistry and Molecular Pharmacology
Fluids and Secretions | Hemic and Immune Systems | Investigative Techniques | Laboratory and Basic Science Research | Pharmaceutical Preparations
BACKGROUND AND OBJECTIVE: Based on previous experience of sorbent-mediated ticagrelor, dabigatran, and radiocontrast agent removal, we set out in this study to test the effect of two sorbents on the removal of edoxaban, a factor Xa antagonist direct oral anticoagulant.
METHODS: We circulated 100 mL of edoxaban solution during six first-pass cycles through 40-mL sorbent columns (containing either CytoSorb in three passes or Porapak Q 50-80 mesh in the remaining three passes) during experiments using human plasma and 4% bovine serum albumin solution as drug vehicles. Drug concentration was measured by liquid chromatography-tandem mass spectrometry.
RESULTS: Edoxaban concentration in two experiments performed with human plasma dropped from 276.8 to 2.7 ng/mL and undetectable concentrations, respectively, with CytoSorb or Porapak Q 50-80 mesh (p = 0.0031). The average edoxaban concentration decreased from 407 ng/mL +/- 216 ng/mL to 3.3 ng/mL +/- 7 ng/mL (p = 0.017), for a removal rate of 99% across all six samples of human plasma (two samples) and bovine serum albumin solution (four samples). In four out of the six adsorbed samples, the drug concentrations were undetectable.
CONCLUSION: Sorbent-mediated technology may represent a viable pathway for edoxaban removal from human plasma or albumin solution.
Porapak Q 50–80 mesh, CytoSorb, sorbents, edoxaban, plasma, albumin
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DOI of Published Version
Angheloiu AA, Tan Y, Ruse C, Shaffer SA, Angheloiu GO. In-Vitro Sorbent-Mediated Removal of Edoxaban from Human Plasma and Albumin Solution. Drugs R D. 2020 Sep;20(3):217-223. doi: 10.1007/s40268-020-00308-1. PMID: 32415538; PMCID: PMC7419416. Link to article on publisher's site
Drugs in R and D
Angheloiu AA, Tan Y, Ruse C, Shaffer SA, Angheloiu GO. (2020). In-Vitro Sorbent-Mediated Removal of Edoxaban from Human Plasma and Albumin Solution. Open Access Publications by UMMS Authors. https://doi.org/10.1007/s40268-020-00308-1. Retrieved from https://escholarship.umassmed.edu/oapubs/4354
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License