Department of Microbiology and Physiological Systems; Department of Biochemistry and Molecular Pharmacology
Amino Acids, Peptides, and Proteins | Biochemistry, Biophysics, and Structural Biology | Biological Factors | Immunology and Infectious Disease | Lipids | Microbiology | Virus Diseases | Viruses
Lassa virus (LASV) is the causative agent of Lassa hemorrhagic fever, a lethal disease endemic to Western Africa. LASV entry is mediated by the viral envelope glycoprotein (GP), a class I membrane fusogen and the sole viral surface antigen. Previous studies have identified components of the LASV entry pathway, including several cellular receptors and the requirement of endosomal acidification for infection. Here, we first demonstrate that incubation at a physiological temperature and pH consistent with the late endosome is sufficient to render pseudovirions, bearing LASV GP, non-infectious. Antibody binding indicates that this loss of infectivity is due to a conformational change in GP. Finally, we developed a single-particle fluorescence assay to directly visualize individual pseudovirions undergoing LASV GP-mediated lipid mixing with a supported planar bilayer. We report that exposure to endosomal pH at a physiologic temperature is sufficient to trigger GP-mediated lipid mixing. Furthermore, while a cellular receptor is not necessary to trigger lipid mixing, the presence of lysosomal-associated membrane protein 1 (LAMP1) increases the kinetics of lipid mixing at an endosomal pH. Furthermore, we find that LAMP1 permits robust lipid mixing under less acidic conditions than in its absence. These findings clarify our understanding of LASV GP-mediated fusion and the role of LAMP1 binding.
Lassa virus, membrane fusion, single-particle fusion, virus entry
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DOI of Published Version
Bulow U, Govindan R, Munro JB. Acidic pH Triggers Lipid Mixing Mediated by Lassa Virus GP. Viruses. 2020 Jul 2;12(7):E716. doi: 10.3390/v12070716. PMID: 32630688. Link to article on publisher's site
Bulow U, Govindan R, Munro JB. (2020). Acidic pH Triggers Lipid Mixing Mediated by Lassa Virus GP. Open Access Publications by UMMS Authors. https://doi.org/10.3390/v12070716. Retrieved from https://escholarship.umassmed.edu/oapubs/4302
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