UMMS Affiliation

Program in Molecular Medicine

Publication Date

2020-07-07

Document Type

Article

Disciplines

Animal Experimentation and Research | Research Methods in Life Sciences | Translational Medical Research

Abstract

Mice xenotransplanted with human cells and/or expressing human gene products (also known as "humanized mice") recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human-specific physiology and pathologies. Humanized mice have advanced toward mainstream preclinical models and are now at the forefront of biomedical research. Here, we considered innovations and challenges regarding the reconstitution of human immunity and human tissues, modeling of human infections and cancer, and the use of humanized mice for testing drugs or regenerative therapy products. As the number of publications exploring different facets of humanized mouse models has steadily increased in past years, it is becoming evident that standardized reporting is needed in the field. Therefore, an international community-driven resource called "Minimal Information for Standardization of Humanized Mice" (MISHUM) has been created for the purpose of enhancing rigor and reproducibility of studies in the field. Within MISHUM, we propose comprehensive guidelines for reporting critical information generated using humanized mice.

Keywords

PDX, humanized mice, immuno-oncology, infections, regenerative medicine

Rights and Permissions

Copyright 2020 The Authors. Published under the terms of the CC BY 4.0 license.

DOI of Published Version

10.15252/emmm.201708662

Source

Stripecke R, Münz C, Schuringa JJ, Bissig KD, Soper B, Meeham T, Yao LC, Di Santo JP, Brehm M, Rodriguez E, Wege AK, Bonnet D, Guionaud S, Howard KE, Kitchen S, Klein F, Saeb-Parsy K, Sam J, Sharma AD, Trumpp A, Trusolino L, Bult C, Shultz L. Innovations, challenges, and minimal information for standardization of humanized mice. EMBO Mol Med. 2020 Jul 7;12(7):e8662. doi: 10.15252/emmm.201708662. Epub 2020 Jun 24. PMID: 32578942; PMCID: PMC7338801. Link to article on publisher's site

Journal/Book/Conference Title

EMBO molecular medicine

Comments

Full author list omitted for brevity. For the full list of authors, see article.

Related Resources

Link to Article in PubMed

PubMed ID

32578942

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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