Program in Molecular Medicine
Animal Experimentation and Research | Research Methods in Life Sciences | Translational Medical Research
Mice xenotransplanted with human cells and/or expressing human gene products (also known as "humanized mice") recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human-specific physiology and pathologies. Humanized mice have advanced toward mainstream preclinical models and are now at the forefront of biomedical research. Here, we considered innovations and challenges regarding the reconstitution of human immunity and human tissues, modeling of human infections and cancer, and the use of humanized mice for testing drugs or regenerative therapy products. As the number of publications exploring different facets of humanized mouse models has steadily increased in past years, it is becoming evident that standardized reporting is needed in the field. Therefore, an international community-driven resource called "Minimal Information for Standardization of Humanized Mice" (MISHUM) has been created for the purpose of enhancing rigor and reproducibility of studies in the field. Within MISHUM, we propose comprehensive guidelines for reporting critical information generated using humanized mice.
PDX, humanized mice, immuno-oncology, infections, regenerative medicine
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Copyright 2020 The Authors. Published under the terms of the CC BY 4.0 license.
DOI of Published Version
Stripecke R, Münz C, Schuringa JJ, Bissig KD, Soper B, Meeham T, Yao LC, Di Santo JP, Brehm M, Rodriguez E, Wege AK, Bonnet D, Guionaud S, Howard KE, Kitchen S, Klein F, Saeb-Parsy K, Sam J, Sharma AD, Trumpp A, Trusolino L, Bult C, Shultz L. Innovations, challenges, and minimal information for standardization of humanized mice. EMBO Mol Med. 2020 Jul 7;12(7):e8662. doi: 10.15252/emmm.201708662. Epub 2020 Jun 24. PMID: 32578942; PMCID: PMC7338801. Link to article on publisher's site
EMBO molecular medicine
Stripecke R, Brehm MA. (2020). Innovations, challenges, and minimal information for standardization of humanized mice. Open Access Articles. https://doi.org/10.15252/emmm.201708662. Retrieved from https://escholarship.umassmed.edu/oapubs/4291
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This work is licensed under a Creative Commons Attribution 4.0 License.