CD11cHi monocyte-derived macrophages are a major cellular compartment infected by Mycobacterium tuberculosis
Authors
Lee, JinheeBoyce, Shayla
Powers, Jennifer
Baer, Christina E.
Sassetti, Christopher M.
Behar, Samuel M.
UMass Chan Affiliations
Graduate School of Biomedical SciencesDepartment of Microbiology and Physiological Systems
Document Type
Journal ArticlePublication Date
2020-06-16Keywords
Bone marrow cellsMycobacterium tuberculosis
Cloning
Macrophages
T cells
Monocytes
Gene expression
Respiratory infections
Bacterial Infections and Mycoses
Immunity
Immunology of Infectious Disease
Immunopathology
Pathogenic Microbiology
Metadata
Show full item recordAbstract
During tuberculosis, lung myeloid cells have two opposing roles: they are an intracellular niche occupied by Mycobacterium tuberculosis, and they restrict bacterial replication. Lung myeloid cells from mice infected with yellow-fluorescent protein expressing M. tuberculosis were analyzed by flow cytometry and transcriptional profiling to identify the cell types infected and their response to infection. CD14, CD38, and Abca1 were expressed more highly by infected alveolar macrophages and CD11cHi monocyte-derived cells compared to uninfected cells. CD14, CD38, and Abca1 "triple positive" (TP) cells had not only the highest infection rates and bacterial loads, but also a strong interferon-gamma signature and nitric oxide synthetase-2 production indicating recognition by T cells. Despite evidence of T cell recognition and appropriate activation, these TP macrophages are a cellular compartment occupied by M. tuberculosis long-term. Defining the niche where M. tuberculosis resists elimination promises to provide insight into why inducing sterilizing immunity is a formidable challenge.Source
Lee J, Boyce S, Powers J, Baer C, Sassetti CM, Behar SM. CD11cHi monocyte-derived macrophages are a major cellular compartment infected by Mycobacterium tuberculosis. PLoS Pathog. 2020 Jun 16;16(6):e1008621. doi: 10.1371/journal.ppat.1008621. PMID: 32544188; PMCID: PMC7319360. Link to article on publisher's site
DOI
10.1371/journal.ppat.1008621Permanent Link to this Item
http://hdl.handle.net/20.500.14038/41499PubMed ID
32544188Related Resources
Rights
Copyright: © 2020 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1371/journal.ppat.1008621
Scopus Count
Except where otherwise noted, this item's license is described as Copyright: © 2020 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.