School of Medicine
Bacterial Infections and Mycoses | Diagnosis | Health Services Administration | Health Services Research | International Public Health | Microbiology
BACKGROUND: There is a well-documented lack of rapid, low-cost tuberculosis (TB) drug resistance diagnostics in low-income settings across the globe. It is these areas that are plagued with a disproportionately high disease burden and in greatest need of these diagnostics.
METHODS: In this study, we compared the performance of Light Forge, a microfluidic high-resolution melting analysis (HRMA) prototype for rapid low-cost detection of TB drug resistance with a commercial HRMA device, a predictive "nearest-neighbor" thermodynamic model, DNA sequencing, and phenotypic drug susceptibility testing (DST). The initial development and assessment of the Light Forge assay was performed with 7 phenotypically drug resistant strains of Mycobacterium tuberculosis (M.tb) that had their rpoB gene subsequently sequenced to confirm resistance to Rifampin. These isolates of M.tb were then compared against a drug-susceptible standard, H37Rv. Seven strains of M.tb were isolated from clinical specimens and individually analyzed to characterize the unique melting profile of each strain.
RESULTS: Light Forge was able to detect drug-resistance linked mutations with 100% concordance to the sequencing, phenotypic DST and the "nearest neighbor" thermodynamic model. Researchers were then blinded to the resistance profile of the seven M.tb strains. In this experiment, Light Forge correctly classified 7 out of 9 strains as either drug resistant or drug susceptible.
CONCLUSIONS: Light Forge represents a promising prototype for a fast, low-cost diagnostic alternative for detection of drug resistant strains of TB in resource constrained settings.
Mycobacterium tuberculosis, Drug resistance, High-resolution melting analysis, Molecular diagnostics, Real-time PCR
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Copyright American Association for Clinical Chemistry 2020. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creative commons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact email@example.com
DOI of Published Version
Mbano IM, Mandizvo T, Rogich J, Kunota TTR, Mackenzie JS, Pillay M, Balagaddé FK. Light Forge: A Microfluidic DNA Melting-based Tuberculosis Test. J Appl Lab Med. 2020 May 1;5(3):440-453. doi: 10.1093/jalm/jfaa019. PMID: 32445364; PMCID: PMC7192548. Link to article on publisher's site
The journal of applied laboratory medicine
Mbano IM, Mandizvo T, Rogich J, Kunota TT, Mackenzie JS, Pillay M, Balagadde FK. (2020). Light Forge: A Microfluidic DNA Melting-based Tuberculosis Test. Open Access Publications by UMMS Authors. https://doi.org/10.1093/jalm/jfaa019. Retrieved from https://escholarship.umassmed.edu/oapubs/4246
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.