UMMS Affiliation

Program in Molecular Medicine; Program in Bioinformatics and Integrative Biology; Graduate School of Biomedical Sciences

Publication Date

2020-02-25

Document Type

Article

Disciplines

Bioinformatics | Ecology and Evolutionary Biology | Genetic Phenomena | Genomics | Integrative Biology | Investigative Techniques | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

In Drosophila, transposon-silencing piRNAs are derived from heterochromatic clusters and a subset of euchromatic transposon insertions, which are bound by the Rhino-Deadlock-Cutoff complex. The HP1 homolog Rhino binds to Deadlock, which recruits TRF2 to promote non-canonical transcription from both genomic strands. Cuff function is less well understood, but this Rai1 homolog shows hallmarks of adaptive evolution, which can remodel functional interactions within host defense systems. Supporting this hypothesis, Drosophila simulans Cutoff is a dominant-negative allele when expressed in Drosophila melanogaster, in which it traps Deadlock, TRF2, and the conserved transcriptional co-repressor CtBP in stable complexes. Cutoff functions with Rhino and Deadlock to drive non-canonical transcription. In contrast, CtBP suppresses canonical transcription of transposons and promoters flanking the major germline clusters, and canonical transcription interferes with downstream non-canonical transcription and piRNA production. Adaptive evolution thus targets interactions among Cutoff, TRF2, and CtBP that balance canonical and non-canonical piRNA precursor transcription.

Keywords

CtBP, Cutoff, TRF2, adaptive evolution, cross-species complementation, piRNA cluster transcriptional regulation, piRNA pathway, transposon silencing

Rights and Permissions

Copyright 2020 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

DOI of Published Version

10.1016/j.celrep.2020.01.109

Source

Parhad SS, Yu T, Zhang G, Rice NP, Weng Z, Theurkauf WE. Adaptive Evolution Targets a piRNA Precursor Transcription Network. Cell Rep. 2020 Feb 25;30(8):2672-2685.e5. doi: 10.1016/j.celrep.2020.01.109. PMID: 32101744; PMCID: PMC7061269. Link to article on publisher's site

Journal/Book/Conference Title

Cell reports

Related Resources

Link to Article in PubMed

PubMed ID

32101744

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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