UMMS Affiliation

Department of Urology

Publication Date

2020-01-02

Document Type

Article Postprint

Disciplines

Amino Acids, Peptides, and Proteins | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Bacterial Infections and Mycoses | Biochemistry | Female Urogenital Diseases and Pregnancy Complications | Male Urogenital Diseases | Molecular Biology | Pediatrics | Urology

Abstract

Recurrent urinary tract infections (UTIs) pose a significant burden on the health care system. Underlying mechanisms predisposing children to UTIs and associated changes in the urinary proteome are not well understood. We aimed to investigate the urinary proteome of a subset of children who have vesicoureteral reflux (VUR) and recurrent UTIs because of their risk of developing infection-related renal damage. Improving diagnostic modalities to identify UTI risk factors would significantly alter the clinical management of children with VUR. We profiled the urinary proteomes of 22 VUR patients with low grade VUR (1-3 out of 5), a history of recurrent UTIs, and renal scarring, comparing them to those obtained from 22 age-matched controls. Urinary proteins were analyzed by mass spectrometry followed by protein quantitation based on spectral counting. Of the 2,551 proteins identified across both cohorts, 964 were robustly quantified, as defined by meeting criteria with spectral count (SC) > /=2 in at least 7 patients in either VUR or control cohort based on optimization of signal-to-noise ratio. Eighty proteins had differential expression between the two cohorts, with 44 proteins significantly upregulated and 36 downregulated (q < 0.075, |FC| > 1.2). Urinary proteins involved in inflammation, acute phase response (APR), modulation of extracellular matrix (ECM), and carbohydrate metabolism were overrepresented among the study cohort.

Keywords

Clinical proteomics, Infectious disease, Mass Spectrometry, Renal function or biology, Urine analysis, acute phase response, urinary tract infection, vesicoureteral reflux

Rights and Permissions

This research was originally published in Molecular & Cellular Proteomics. Vitko D et al. Characterizing Patients with Recurrent Urinary Tract Infections in Vesicoureteral Reflux: A Pilot Study of the Urinary Proteome. Mol Cell Proteomics. 2020 Jan 2:mcp.RA119.001873. doi: 10.1074/mcp.RA119.001873. © the Author(s). Accepted manuscript posted as allowed by publisher's copyright policy at https://www.mcponline.org/content/editorial-policies-and-practices.

DOI of Published Version

10.1074/mcp.RA119.001873

Source

Vitko D, Cho P, Kostel SA, DiMartino SE, Cabour LD, Migliozzi MA, Logvinenko T, Warren PG, Froehlich JW, Lee RS. Characterizing Patients with Recurrent Urinary Tract Infections in Vesicoureteral Reflux: A Pilot Study of the Urinary Proteome. Mol Cell Proteomics. 2020 Jan 2:mcp.RA119.001873. doi: 10.1074/mcp.RA119.001873. Epub ahead of print. PMID: 31896675. Link to article on publisher's site

Journal/Book/Conference Title

Molecular and cellular proteomics : MCP

Related Resources

Link to Article in PubMed

PubMed ID

31896675

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