UMMS Affiliation

Program in Innate Immunity, Division of Infectious Diseases and Immunology, Department of Medicine

Publication Date

2020-01-22

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Bacteria | Bacterial Infections and Mycoses | Bacteriology | Immunity | Immunopathology | Immunoprophylaxis and Therapy | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

Long non-coding RNAs (lncRNAs) have emerged as key regulators of gene expression in several biological systems. The long intergenic RNA-erythroid pro-survival (lincRNA-EPS) has been shown to play a critical role in restraining inflammatory gene expression. However, the function of lincRNA-EPS during bacterial infections remains unknown. Here, we demonstrate that following infection with the intracellular bacterium Listeria monocytogenes, both mouse macrophages and dendritic cells lacking lincRNA-EPS exhibit an enhanced expression of proinflammatory cytokine genes, as well as an increased expression of the inducible nitric oxide synthase (iNos) and nitric oxide (NO) production. Importantly, we found that lincRNA-EPS(-/-) mice intraperitoneally infected with L. monocytogenes exhibit lower bacterial burdens in spleen and liver and produce more NO than control mice. Furthermore, lincRNA-EPS(-/-) mice are less susceptible to a lethal dose of L. monocytogenes than wild type (WT) mice. Collectively these findings show that lincRNA-EPS suppresses host protective NO expression and impairs the host defense against L. monocytogenes infection.

Keywords

Listeria monocytogenes, infection, innate immunity, lincRNA-EPS, lncRNAs

Rights and Permissions

Copyright © 2020 Agliano, Fitzgerald, Vella, Rathinam and Medvedev. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

DOI of Published Version

10.3389/fcimb.2019.00481

Source

Agliano F, Fitzgerald KA, Vella AT, Rathinam VA, Medvedev AE. Long Non-coding RNA LincRNA-EPS Inhibits Host Defense Against Listeria monocytogenes Infection. Front Cell Infect Microbiol. 2020 Jan 22;9:481. doi: 10.3389/fcimb.2019.00481. PMID: 32039056; PMCID: PMC6987077. Link to article on publisher's site

Journal/Book/Conference Title

Frontiers in cellular and infection microbiology

Related Resources

Link to Article in PubMed

PubMed ID

32039056

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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