UMMS Affiliation

RNA Therapeutics Institute

Publication Date

2020-02-01

Document Type

Article

Disciplines

Analytical, Diagnostic and Therapeutic Techniques and Equipment | Genetics and Genomics | Laboratory and Basic Science Research | Nanomedicine | Nervous System | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

Data on preparation and characterization of chitosan-based nanoparticles (NP) carrying small interfering RNA (siRNA) for non-invasive gene therapy is presented. Polyelectrolyte complexation method was carried out in diluted concentrations to obtain relatively small (less than 200 nm) NP. To provide substantial dose of siRNA within tolerable volume of intranasal administration the NP were subjected to enrichment process. Offered here NP fabrication does two steps process comprise provisional and enriched preparations? The differences between these preparations were analyzed with hydrodynamic size distribution and zeta potential measurements. The effect of siRNA lipophilicity on NP physical instability was also tested. Biological evaluation of nanoparticles is described in our published article [1].

Keywords

Chitosan, Enrichment, Nanocarriers, Polyelectrolyte complexation, siRNA

Rights and Permissions

© 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

DOI of Published Version

10.1016/j.dib.2019.105093

Source

Sava V, Fihurka O, Khvorova A, Sanchez-Ramos J. Data on enrichment of chitosan nanoparticles for intranasal delivery of oligonucleotides to the brain. Data Brief. 2020 Jan 3;28:105093. doi: 10.1016/j.dib.2019.105093. PMID: 31970274; PMCID: PMC6965707. Link to article on publisher's site

Journal/Book/Conference Title

Data in brief

Related Resources

Link to Article in PubMed

PubMed ID

31970274

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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