UMMS Affiliation
Department of Neurobiology; Emery Lab; Graduate School of Biomedical Sciences
Publication Date
2019-11-08
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Neuroscience and Neurobiology | Nucleic Acids, Nucleotides, and Nucleosides
Abstract
The Drosophila circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studied in detail, much less is known about circadian post-transcriptional control. Thus, we targeted 364 RNA binding and RNA associated proteins with RNA interference. Among the 43 hits we identified was the alternative splicing regulator P-element somatic inhibitor (PSI). PSI regulates the thermosensitive alternative splicing of timeless (tim), promoting splicing events favored at warm temperature over those increased at cold temperature. Psi downregulation shortens the period of circadian rhythms and advances the phase of circadian behavior under temperature cycle. Interestingly, both phenotypes were suppressed in flies that could produce TIM proteins only from a transgene that cannot form the thermosensitive splicing isoforms. Therefore, we conclude that PSI regulates the period of Drosophila circadian rhythms and circadian behavior phase during temperature cycling through its modulation of the tim splicing pattern.
Keywords
D. melanogaster, RNA binding proteins, alternative splicing, circadian rhythms, neuroscience, timeless
Rights and Permissions
Copyright Foley et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI of Published Version
10.7554/eLife.50063
Source
Elife. 2019 Nov 8;8. pii: 50063. doi: 10.7554/eLife.50063. Link to article on publisher's site
Journal/Book/Conference Title
eLife
Related Resources
PubMed ID
31702555
Repository Citation
Foley LE, Ling J, Joshi RS, Evantal N, Kadener S, Emery P. (2019). Drosophila PSI controls circadian period and the phase of circadian behavior under temperature cycle via tim splicing. Open Access Articles. https://doi.org/10.7554/eLife.50063. Retrieved from https://escholarship.umassmed.edu/oapubs/4059
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Neuroscience and Neurobiology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons