UMMS Affiliation

Department of Molecular, Cell and Cancer Biology

Publication Date

2019-11-06

Document Type

Article

Disciplines

Cell Biology | Cellular and Molecular Physiology | Lipids | Nutritional and Metabolic Diseases | Physiological Processes

Abstract

Aging usually involves the progressive development of certain illnesses, including diabetes and obesity. Due to incapacity to form new white adipocytes, adipose expansion in aged mice primarily depends on adipocyte hypertrophy, which induces metabolic dysfunction. On the other hand, brown adipose tissue burns fatty acids, preventing ectopic lipid accumulation and metabolic diseases. However, the capacity of brown/beige adipogenesis declines inevitably during the aging process. Previously, we reported that DNA demethylation in the Prdm16 promoter is required for beige adipogenesis. DNA methylation is mediated by ten-eleven family proteins (TET) using alpha-ketoglutarate (AKG) as a cofactor. Here, we demonstrated that the circulatory AKG concentration was reduced in middle-aged mice (10-month-old) compared with young mice (2-month-old). Through AKG administration replenishing the AKG pool, aged mice were associated with the lower body weight gain and fat mass, and improved glucose tolerance after challenged with high-fat diet (HFD). These metabolic changes are accompanied by increased expression of brown adipose genes and proteins in inguinal adipose tissue. Cold-induced brown/beige adipogenesis was impeded in HFD mice, whereas AKG rescued the impairment of beige adipocyte functionality in middle-aged mice. Besides, AKG administration up-regulated Prdm16 expression, which was correlated with an increase of DNA demethylation in the Prdm16 promoter. In summary, AKG supplementation promotes beige adipogenesis and alleviates HFD-induced obesity in middle-aged mice, which is associated with enhanced DNA demethylation of the Prdm16 gene.

Keywords

DNA demethylation, adipose tissue browning, aging, alpha-ketoglutarate, glucose tolerance, high-fat diet

Rights and Permissions

© 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley and Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

DOI of Published Version

10.1111/acel.13059

Source

Aging Cell. 2019 Nov 6:e13059. doi: 10.1111/acel.13059. [Epub ahead of print] Link to article on publisher's site

Journal/Book/Conference Title

Aging cell

Related Resources

Link to Article in PubMed

PubMed ID

31691468

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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