UMMS Affiliation

Horae Gene Therapy Center; Department of Microbiology and Physiological Systems; Li Weibo Institute for Rare Diseases Research

Publication Date

2019-09-04

Document Type

Article

Disciplines

Analytical, Diagnostic and Therapeutic Techniques and Equipment | Genetics and Genomics | Research Methods in Life Sciences | Viruses

Abstract

Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno-associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV-based method can facilitate gene function studies in early-stage embryos.

Keywords

CRISPR, adeno‐associated virus (AAV), animal modeling, genome editing

Rights and Permissions

© 2019 The Authors. Published by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

DOI of Published Version

10.1002/advs.201900440

Source

Adv Sci (Weinh). 2019 Sep 4;6(21):1900440. doi: 10.1002/advs.201900440. eCollection 2019 Nov 6. Link to article on publisher's site

Journal/Book/Conference Title

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Related Resources

Link to Article in PubMed

PubMed ID

31728271

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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