UMMS Affiliation

RNA Therapeutics Institute

Publication Date

2019-10-18

Document Type

Article

Disciplines

Biochemical Phenomena, Metabolism, and Nutrition | Computational Biology | Dietetics and Clinical Nutrition | Molecular, Genetic, and Biochemical Nutrition | Plant Sciences

Abstract

Snap beans are a significant source of micronutrients in the human diet. Among the micronutrients present in snap beans are phenolic compounds with known beneficial effects on human health, potentially via their metabolism by the gut-associated microbiome. The genetic pathways leading to the production of phenolics in snap bean pods remain uncertain. In this study, we quantified the level of total phenolic content (TPC) in the Bean Coordinated Agriculture Program (CAP) snap bean diversity panel of 149 accessions. The panel was characterized spectrophotometrically for phenolic content with a Folin-Ciocalteu colorimetric assay. Flower, seed and pod color were also quantified, as red, purple, yellow and brown colors are associated with anthocyanins and flavonols in common bean. Genotyping was performed through an Illumina Infinium Genechip BARCBEAN6K_3 single nucleotide polymorphism (SNP) array. Genome-Wide Association Studies (GWAS) analysis identified 11 quantitative trait nucleotides (QTN) associated with TPC. An SNP was identified for TPC on Pv07 located near the P gene, which is a major switch in the flavonoid biosynthetic pathway. Candidate genes were identified for seven of the 11 TPC QTN. Five regulatory genes were identified and represent novel sources of variation for exploitation in developing snap beans with higher phenolic levels for greater health benefits to the consumer.

Keywords

common bean, Phaseolus vulgaris, flavonoids, association mapping, total phenolic compounds, genome wide association mapping

Rights and Permissions

Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

DOI of Published Version

10.3390/nu11102509

Source

Nutrients. 2019 Oct 18;11(10). pii: nu11102509. doi: 10.3390/nu11102509. Link to article on publisher's site

Journal/Book/Conference Title

Nutrients

Related Resources

Link to Article in PubMed

PubMed ID

31635241

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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