UMMS Affiliation
RNA Therapeutics Institute
Publication Date
2019-10-18
Document Type
Article
Disciplines
Biochemical Phenomena, Metabolism, and Nutrition | Computational Biology | Dietetics and Clinical Nutrition | Molecular, Genetic, and Biochemical Nutrition | Plant Sciences
Abstract
Snap beans are a significant source of micronutrients in the human diet. Among the micronutrients present in snap beans are phenolic compounds with known beneficial effects on human health, potentially via their metabolism by the gut-associated microbiome. The genetic pathways leading to the production of phenolics in snap bean pods remain uncertain. In this study, we quantified the level of total phenolic content (TPC) in the Bean Coordinated Agriculture Program (CAP) snap bean diversity panel of 149 accessions. The panel was characterized spectrophotometrically for phenolic content with a Folin-Ciocalteu colorimetric assay. Flower, seed and pod color were also quantified, as red, purple, yellow and brown colors are associated with anthocyanins and flavonols in common bean. Genotyping was performed through an Illumina Infinium Genechip BARCBEAN6K_3 single nucleotide polymorphism (SNP) array. Genome-Wide Association Studies (GWAS) analysis identified 11 quantitative trait nucleotides (QTN) associated with TPC. An SNP was identified for TPC on Pv07 located near the P gene, which is a major switch in the flavonoid biosynthetic pathway. Candidate genes were identified for seven of the 11 TPC QTN. Five regulatory genes were identified and represent novel sources of variation for exploitation in developing snap beans with higher phenolic levels for greater health benefits to the consumer.
Keywords
common bean, Phaseolus vulgaris, flavonoids, association mapping, total phenolic compounds, genome wide association mapping
Rights and Permissions
Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI of Published Version
10.3390/nu11102509
Source
Nutrients. 2019 Oct 18;11(10). pii: nu11102509. doi: 10.3390/nu11102509. Link to article on publisher's site
Journal/Book/Conference Title
Nutrients
Related Resources
PubMed ID
31635241
Repository Citation
Myers JR, Wallace LT, Mafi Moghaddam S, Kleintop AE, Echeverria D, Thompson HJ, Brick MA, Lee R, McClean PE. (2019). Improving the Health Benefits of Snap Bean: Genome-Wide Association Studies of Total Phenolic Content. Open Access Articles. https://doi.org/10.3390/nu11102509. Retrieved from https://escholarship.umassmed.edu/oapubs/4025
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Biochemical Phenomena, Metabolism, and Nutrition Commons, Computational Biology Commons, Dietetics and Clinical Nutrition Commons, Molecular, Genetic, and Biochemical Nutrition Commons, Plant Sciences Commons