UMMS Affiliation
Department of Molecular, Cell and Cancer Biology
Publication Date
2019-10-24
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Cell Biology | Cells | Developmental Biology | Nucleic Acids, Nucleotides, and Nucleosides
Abstract
MicroRNAs modulate a variety of cellular events, including skeletal muscle development, but the molecular basis of their functions in fetal bovine skeletal muscle development is poorly understood. In this study, we report that bta-miR-24-3p promotes the myogenic differentiation of fetal bovine PDGFRalpha(-) progenitor cells. The expression of bta-miR-24-3p increased during myogenic differentiation. Overexpression of bta-miR-24-3p significantly promoted myogenic differentiation, but inhibited proliferation. A dual-luciferase assay identified ACVR1B as a direct target of bta-miR-24-3p. Similarly, knocking down ACVR1B by RNA interference also significantly inhibited proliferation and promoted the differentiation of bovine PDGFRalpha(-) progenitor cells. Thus, our study provides a mechanism in which bta-miR-24-3p regulates myogenesis by inhibiting ACVR1B expression.
Keywords
bta-miR-24-3p, bovine, fetal skeletal muscle, proliferation, differentiation, ACVR1B
Rights and Permissions
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI of Published Version
10.3390/ani9110859
Source
Animals (Basel). 2019 Oct 24;9(11). pii: ani9110859. doi: 10.3390/ani9110859. Link to article on publisher's site
Journal/Book/Conference Title
Animals : an open access journal from MDPI
Related Resources
PubMed ID
31652908
Repository Citation
Hu X, Xing Y, Ren L, Wang Y, Li Q, Fu X, Yang Q, Xu L, Willems L, Li J, Zhang L. (2019). Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal, Bovine, Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B. Open Access Publications by UMass Chan Authors. https://doi.org/10.3390/ani9110859. Retrieved from https://escholarship.umassmed.edu/oapubs/4005
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
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