UMMS Affiliation
Department of Medicine, Division of Cardiovascular Medicine; Department of Population and Quantitative Health Sciences; Department of Pediatrics, Division of Pulmonary Medicine; Horae Gene Therapy Center
Publication Date
2019-09-27
Document Type
Article
Disciplines
Cardiovascular Diseases | Genetics and Genomics | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides | Population Biology | Respiratory System | Respiratory Tract Diseases
Abstract
The presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence of these pine-pollen miRNAs associated with hay fever and not with overt cardiovascular or pulmonary disease. Using in vivo and in vitro models, we found that transmission of pollen-miRNAs into the circulation occurs via pulmonary transfer and this transfer was mediated by platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake. These data demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans. Although these data suggest mechanistic plausibility for pulmonary-mediated plant-derived miRNA transfer into the human circulation, our large observational cohort data do not implicate major disease or risk factor association.
Keywords
Biological Sciences, Molecular Biology, Omics, Transcriptomics
Rights and Permissions
Copyright 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI of Published Version
10.1016/j.isci.2019.08.035
Source
iScience. 2019 Sep 27;19:916-926. doi: 10.1016/j.isci.2019.08.035. Epub 2019 Aug 24. Link to article on publisher's site
Journal/Book/Conference Title
iScience
Related Resources
PubMed ID
31518900
Repository Citation
Koupenova-Zamor M, Mick EO, Corkrey HA, Singh A, Tanriverdi SE, Vitseva O, Levy D, Keeler AM, Ezzaty Mirhashemi M, Elmallah MK, Gerstein M, Rozowsky J, Tanriverdi K, Freedman JE. (2019). Pollen-derived RNAs Are Found in the Human Circulation. Open Access Articles. https://doi.org/10.1016/j.isci.2019.08.035. Retrieved from https://escholarship.umassmed.edu/oapubs/3983
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Cardiovascular Diseases Commons, Genetics and Genomics Commons, Molecular Biology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons, Population Biology Commons, Respiratory System Commons, Respiratory Tract Diseases Commons