Department of Molecular, Cell and Cancer Biology
Amino Acids, Peptides, and Proteins | Cell Biology | Cellular and Molecular Physiology | Lipids
Lipoproteins are lipid-protein complexes that are primarily generated and secreted from the intestine, liver, and visceral endoderm and delivered to peripheral tissues. Lipoproteins, which are assembled in the endoplasmic reticulum (ER) membrane, are released into the ER lumen for secretion, but its mechanism remains largely unknown. Here, we show that the release of lipoproteins from the ER membrane requires VMP1, an ER transmembrane protein essential for autophagy and certain types of secretion. Loss of vmp1, but not other autophagy-related genes, in zebrafish causes lipoprotein accumulation in the intestine and liver. Vmp1 deficiency in mice also leads to lipid accumulation in the visceral endoderm and intestine. In VMP1-depleted cells, neutral lipids accumulate within lipid bilayers of the ER membrane, thus affecting lipoprotein secretion. These results suggest that VMP1 is important for the release of lipoproteins from the ER membrane to the ER lumen in addition to its previously known functions.
VMP1, autophagy-related gene, cell biology, endoplasmic reticulum, human, lipoprotein, mouse, zebrafish
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Copyright © 2019, Morishita et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI of Published Version
Elife. 2019 Sep 17;8. pii: 48834. doi: 10.7554/eLife.48834. Link to article on publisher's site
Morishita H, Zhao YG, Tamura N, Nishimura T, Kanda Y, Sakamaki Y, Okazaki M, Li D, Mizushima N. (2019). A critical role of VMP1 in lipoprotein secretion. Open Access Articles. https://doi.org/10.7554/eLife.48834. Retrieved from https://escholarship.umassmed.edu/oapubs/3977
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This work is licensed under a Creative Commons Attribution 4.0 License.