UMMS Affiliation
RNA Therapeutics Institute; Department of Biochemistry and Molecular Pharmacology
Publication Date
2019-09-12
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Bacteria | Biochemical Phenomena, Metabolism, and Nutrition | Biophysics | Cell Biology | Cells | Nucleic Acids, Nucleotides, and Nucleosides | Structural Biology
Abstract
Protein synthesis ends when a ribosome reaches an mRNA stop codon. Release factors (RFs) decode the stop codon, hydrolyze peptidyl-tRNA to release the nascent protein, and then dissociate to allow ribosome recycling. To visualize termination by RF2, we resolved a cryo-EM ensemble of E. coli 70S*RF2 structures at up to 3.3 A in a single sample. Five structures suggest a highly dynamic termination pathway. Upon peptidyl-tRNA hydrolysis, the CCA end of deacyl-tRNA departs from the peptidyl transferase center. The catalytic GGQ loop of RF2 is rearranged into a long beta-hairpin that plugs the peptide tunnel, biasing a nascent protein toward the ribosome exit. Ribosomal intersubunit rotation destabilizes the catalytic RF2 domain on the 50S subunit and disassembles the central intersubunit bridge B2a, resulting in RF2 departure. Our structures visualize how local rearrangements and spontaneous inter-subunit rotation poise the newly-made protein and RF2 to dissociate in preparation for ribosome recycling.
Keywords
E. coli, GGQ motif rearrangement, cell biology, ensemble cryo-EM, intersubunit rotation, molecular biophysics, release factor 2, ribosome recycling, structural biology, translation termination
Rights and Permissions
Copyright © 2019, Svidritskiy et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI of Published Version
10.7554/eLife.46850
Source
Elife. 2019 Sep 12;8. pii: 46850. doi: 10.7554/eLife.46850. Link to article on publisher's site
Journal/Book/Conference Title
eLife
Related Resources
PubMed ID
31513010
Repository Citation
Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA. (2019). Extensive ribosome and RF2 rearrangements during translation termination. Open Access Articles. https://doi.org/10.7554/eLife.46850. Retrieved from https://escholarship.umassmed.edu/oapubs/3976
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Bacteria Commons, Biochemical Phenomena, Metabolism, and Nutrition Commons, Biophysics Commons, Cell Biology Commons, Cells Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons, Structural Biology Commons