Department of Biochemistry and Molecular Pharmacology; Imbalzano Lab
Amino Acids, Peptides, and Proteins | Biochemistry | Cell Biology | Cellular and Molecular Physiology | Enzymes and Coenzymes | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides | Structural Biology
JMJD6 is a member of the Jumonji C domain containing enzymes that demethylate and/or hydroxylate substrate proteins. It is a multi-functional protein that has been implicated in disparate aspects of transcriptional and post-transcriptional control of gene expression, including but not limited to enhancer and promoter binding, release of paused RNA polymerase II, control of splicing, and interaction with the translation machinery. JMJD6 contributes to multiple aspects of animal development, including adipogenesis modeled in culture. We mutated proposed or characterized domains in the JMJD6 protein to better understand the requirement for JMJD6 in adipogenic differentiation. Mutation of JMJD6 amino acids that mediate binding of iron and 2-oxogluterate, which are required cofactors for enzymatic activity, had no impact on JMJD6 function, showing that catalytic activity is not required for JMJD6 contributions to adipogenic differentiation. In addition, we documented the formation of JMJD6 oligomers and showed that catalytic activity is not required for oligomerization, as has been reported previously. We also observed no effect of mutations in the sumoylation site and in the poly-serine stretch. In contrast, mutation of the AT hook-like structure, which mediates interaction with DNA and/or RNA, compromised JMJD6 function by blocking its ability to interact with chromatin at genes that express regulators of adipogenesis. The ability of JMJD6 to interact with nucleic acids may be a critical requirement for its function in adipogenic differentiation. The requirement for the AT hook-like domain and the lack of requirement for catalytic activity giving rise to the idea that co-activation of transcription by JMJD6 may be functioning as a scaffold protein that supports the interactions of other critical regulators.
Point mutation, Cell differentiation, Chromatin, Gene expression, Oils, Transcriptional control, Protein domains, Sequence motif analysis
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Copyright: © 2019 Reyes-Gutierrez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
PLoS One. 2019 Aug 20;14(8):e0216015. doi: 10.1371/journal.pone.0216015. eCollection 2019. Link to article on publisher's site
Reyes-Gutierrez P, Carrasquillo-Rodriguez JW, Imbalzano AN. (2019). Promotion of adipogenesis by JMJD6 requires the AT hook-like domain and is independent of its catalytic function. Open Access Publications by UMass Chan Authors. https://doi.org/10.1371/journal.pone.0216015. Retrieved from https://escholarship.umassmed.edu/oapubs/3962
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