UMMS Affiliation

Department of Pathology

Publication Date

2019-08-31

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Cancer Biology | Cell Biology | Developmental Biology | Developmental Neuroscience | Enzymes and Coenzymes | Nervous System

Abstract

PSMD10(Gankyrin), a proteasome assembly chaperone, is a widely known oncoprotein which aspects many hall mark properties of cancer. However, except proteasome assembly chaperon function its role in normal cell function remains unknown. To address this issue, we induced PSMD10(Gankyrin) overexpression in HEK293 cells and the resultant large-scale changes in gene expression profile were analyzed. We constituted networks from microarray data of these differentially expressed genes and carried out extensive topological analyses. The overrecurring yet consistent theme that appeared throughout analysis using varied network metrics is that all genes and interactions identified as important would be involved in neurogenesis and neuronal development. Intrigued we tested the possibility that PSMD10(Gankyrin) may be strongly associated with cell fate decisions that commit neural stem cells to differentiate into neurons. Overexpression of PSMD10(Gankyrin) in human neuronal progenitor cells facilitated neuronal differentiation via beta-catenin Ngn1 pathway. Here for the first time we provide preliminary and yet compelling experimental evidence for the involvement of a potential oncoprotein - PSMD10(Gankyrin), in neuronal differentiation.

Keywords

Gankyrin, Human neuronal progenitor cells, Microarray, Neurogenesis, PSMD10, Proteasome

Rights and Permissions

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

DOI of Published Version

10.15283/ijsc19007

Source

Int J Stem Cells. 2019 Aug 31. doi: 10.15283/ijsc19007. [Epub ahead of print] Link to article on publisher's site

Journal/Book/Conference Title

International journal of stem cells

Related Resources

Link to Article in PubMed

PubMed ID

31474027

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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