UMMS Affiliation
Department of Pathology
Publication Date
2019-08-31
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Cancer Biology | Cell Biology | Developmental Biology | Developmental Neuroscience | Enzymes and Coenzymes | Nervous System
Abstract
PSMD10(Gankyrin), a proteasome assembly chaperone, is a widely known oncoprotein which aspects many hall mark properties of cancer. However, except proteasome assembly chaperon function its role in normal cell function remains unknown. To address this issue, we induced PSMD10(Gankyrin) overexpression in HEK293 cells and the resultant large-scale changes in gene expression profile were analyzed. We constituted networks from microarray data of these differentially expressed genes and carried out extensive topological analyses. The overrecurring yet consistent theme that appeared throughout analysis using varied network metrics is that all genes and interactions identified as important would be involved in neurogenesis and neuronal development. Intrigued we tested the possibility that PSMD10(Gankyrin) may be strongly associated with cell fate decisions that commit neural stem cells to differentiate into neurons. Overexpression of PSMD10(Gankyrin) in human neuronal progenitor cells facilitated neuronal differentiation via beta-catenin Ngn1 pathway. Here for the first time we provide preliminary and yet compelling experimental evidence for the involvement of a potential oncoprotein - PSMD10(Gankyrin), in neuronal differentiation.
Keywords
Gankyrin, Human neuronal progenitor cells, Microarray, Neurogenesis, PSMD10, Proteasome
Rights and Permissions
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI of Published Version
10.15283/ijsc19007
Source
Int J Stem Cells. 2019 Aug 31. doi: 10.15283/ijsc19007. [Epub ahead of print] Link to article on publisher's site
Journal/Book/Conference Title
International journal of stem cells
Related Resources
PubMed ID
31474027
Repository Citation
Sahu I, Nanaware PP, Mane M, Mulla SW, Roy S, Venkatraman P. (2019). Role of a 19S Proteasome Subunit- PSMD10(Gankyrin) in Neurogenesis of Human Neuronal Progenitor Cells. Open Access Articles. https://doi.org/10.15283/ijsc19007. Retrieved from https://escholarship.umassmed.edu/oapubs/3950
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Included in
Amino Acids, Peptides, and Proteins Commons, Cancer Biology Commons, Cell Biology Commons, Developmental Biology Commons, Developmental Neuroscience Commons, Enzymes and Coenzymes Commons, Nervous System Commons