Department of Biochemistry and Molecular Pharmacology
Analytical, Diagnostic and Therapeutic Techniques and Equipment | Bioimaging and Biomedical Optics | Cancer Biology | Cell Biology | Cells | Neoplasms
Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.
brain cancer, glioblastoma cells, cancer cells, detection of brain cancer
Rights and Permissions
Copyright © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement, https://www.osapublishing.org/submit/review/pdf/OSACopyTransferOAAgrmnt(2017-09-05).pdf.
DOI of Published Version
Biomed Opt Express. 2019 Jul 29;10(8):4237-4248. doi: 10.1364/BOE.10.004237. eCollection 2019 Aug 1. Link to article on publisher's site
Biomedical optics express
Feng X, Muzikansky A, Ross AH, Hamblin MR, Jermain PR, Yaroslavsky AN. (2019). Multimodal quantitative imaging of brain cancer in cultured cells. Open Access Articles. https://doi.org/10.1364/BOE.10.004237. Retrieved from https://escholarship.umassmed.edu/oapubs/3939