UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

2019-07-29

Document Type

Article

Disciplines

Analytical, Diagnostic and Therapeutic Techniques and Equipment | Bioimaging and Biomedical Optics | Cancer Biology | Cell Biology | Cells | Neoplasms

Abstract

Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.

Keywords

brain cancer, glioblastoma cells, cancer cells, detection of brain cancer

Rights and Permissions

Copyright © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement, https://www.osapublishing.org/submit/review/pdf/OSACopyTransferOAAgrmnt(2017-09-05).pdf.

DOI of Published Version

10.1364/BOE.10.004237

Source

Biomed Opt Express. 2019 Jul 29;10(8):4237-4248. doi: 10.1364/BOE.10.004237. eCollection 2019 Aug 1. Link to article on publisher's site

Journal/Book/Conference Title

Biomedical optics express

Related Resources

Link to Article in PubMed

PubMed ID

31453007

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