UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Department of Pathology

Publication Date

2019-07-17

Document Type

Article

Disciplines

Hemic and Immune Systems | Immune System Diseases | Immunity | Immunopathology | Military and Veterans Studies | Nervous System | Nervous System Diseases | Neuroscience and Neurobiology | Pathological Conditions, Signs and Symptoms | Pharmacology, Toxicology and Environmental Health | Skin and Connective Tissue Diseases

Abstract

Gulf War Illness (GWI), affecting 30% of veterans from the 1991 Gulf War (GW), is a multi-symptom illness with features similar to those of patients with autoimmune diseases. The objective of the current work is to determine if exposure to GW-related pesticides, such as permethrin (PER), activates peripheral and central nervous system (CNS) adaptive immune responses. In the current study, we focused on a PER metabolite, 3-phenoxybenzoic acid (3-PBA), as this is a common metabolite previously shown to form adducts with endogenous proteins. We observed the presence of 3-PBA and 3-PBA modified lysine of protein peptides in the brain, blood and liver of pyridostigmine bromide (PB)and PER (PB+PER) exposed mice at acute and chronic post-exposure timepoints. We tested whether 3-PBA-haptenated albumin (3-PBA-albumin) can activate immune cells since it is known that chemically haptenated proteins can stimulate immune responses. We detected autoantibodies against 3-PBA-albumin in plasma from PB+PER exposed mice and veterans with GWI at chronic post-exposure timepoints. We also observed that in vitro treatment of blood with 3-PBA-albumin resulted in the activation of B- and T-helper lymphocytes and that these immune cells were also increased in blood of PB+PER exposed mice and veterans with GWI. These immune changes corresponded with elevated levels of infiltrating monocytes in the brain and blood of PB+PER exposed mice which coincided with alterations in the markers of blood-brain barrier disruption, brain macrophages and neuroinflammation. These studies suggest that pesticide exposure associated with GWI may have resulted in the activation of the peripheral and CNS adaptive immune responses, possibly contributing to an autoimmune-type phenotype in veterans with GWI.

Keywords

Autoantibody, Gulf War Illness, Hapten, Permethrin, Pesticide

Rights and Permissions

© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).

DOI of Published Version

10.1016/j.bbi.2019.07.015

Source

Brain Behav Immun. 2019 Jul 17. pii: S0889-1591(19)30329-0. doi: 10.1016/j.bbi.2019.07.015. [Epub ahead of print] Link to article on publisher's site

Journal/Book/Conference Title

Brain, behavior, and immunity

Comments

Full author list omitted for brevity. For the full list of authors, see article.

Related Resources

Link to Article in PubMed

PubMed ID

31325531

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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