Title

LY294002, but not wortmannin, increases intracellular calcium and inhibits calcium transients in bovine and human airway smooth muscle cells

UMMS Affiliation

Department of Medicine, Division of Pulmonary, Allergy & Critical Care

Publication Date

2002-07-20

Document Type

Article

Subjects

Androstadienes; Animals; Caffeine; Calcium; Cattle; Chromones; Drug Synergism; Enzyme Inhibitors; Humans; Morpholines; Muscle, Smooth; Phosphodiesterase Inhibitors; Trachea

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured ratiometrically. LY294002 (50 microM) increased [Ca2+]i by 294+/-76 nM (P

Source

Cell Calcium. 2002 Jul;32(1):31-8.

Journal/Book/Conference Title

Cell calcium

Related Resources

Link to article in PubMed

PubMed ID

12127060

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