Department of Biochemistry and Molecular Pharmacology
Amino Acids, Peptides, and Proteins | Cell Biology | Genetics and Genomics | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides | Structural Biology
The genome is packaged and organized in an ordered, non-random manner and specific chromatin segments contact nuclear substructures to mediate this organization. Transfer RNA genes (tDNAs) are binding sites for transcription factors and architectural proteins and are thought to play an important role in the organization of the genome. In this study, we investigate the role of tDNAs in genomic organization and chromosome function by editing a chromosome so that it lacks any tDNAs. Surprisingly our analyses of this tDNA-less chromosome show that loss of tDNAs does not grossly affect chromatin architecture or chromosome tethering and mobility. However, loss of tDNAs affects local nucleosome positioning and the binding of SMC proteins at these loci. The absence of tDNAs also leads to changes in centromere clustering and a reduction in the frequency of long-range HML-HMR heterochromatin clustering with concomitant effects on gene silencing. We propose that the tDNAs primarily affect local chromatin structure that result in effects on long-range chromosome architecture.
SMC proteins, Saccharomyces cerevisiae, chromatin, chromosome structure, gene silencing, nucleosome, tDNA
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This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
DOI of Published Version
Mol Cell Biol. 2019 Apr 2;39(8). pii: e00432-18. doi: 10.1128/MCB.00432-18. Print 2019 Apr 15. Link to article on publisher's site
Molecular and cellular biology
Hamdani O, Hsieh TS, Rando OJ, Kamakaka RT. (2019). Transfer RNA Genes Affect Chromosome Structure and Function via Local Effects. Open Access Publications by UMass Chan Authors. https://doi.org/10.1128/MCB.00432-18. Retrieved from https://escholarship.umassmed.edu/oapubs/3754
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