UMMS Affiliation

Department of Pharmacology

Publication Date

1991-03-01

Document Type

Article

Subjects

DNA; *DNA Glycosylases; Drug Resistance; Ethylnitrosourea; Glioma; N-Glycosyl Hydrolases; Tumor Cells, Cultured

Disciplines

Genetics and Genomics | Medical Pharmacology | Pharmacology

Abstract

Extracts of a glial cell line (SF-126) which is sensitive to the cytotoxic effect of the haloethylnitrosoureas and of a cell line (SF-188) which is resistant to these agents have been tested for their ability to release methylated bases from a DNA substrate which has been modified with [3H]dimethyl sulfate. In comparison with the sensitive cell line, extracts from the resistant cell line have 2-3-fold higher enzymatic activity. High performance liquid chromatography profiles of the bases which are released by these extracts show that the activity is specific for 3-methyladenine, suggesting that the resistant cells contain elevated levels of 3-methyladenine DNA glycosylase. Previous studies have shown that these cells also contain elevated levels of O6-alkylguanine-DNA alkyl-transferase, suggesting that both enzyme activities may be involved in the resistance of this cell line to the haloethylnitrosoureas.

Source

Cancer Res. 1991 Mar 1;51(5):1568-70.

Journal/Book/Conference Title

Cancer research

Related Resources

Link to article in PubMed

PubMed ID

1997198

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