UMMS Affiliation
Program in Molecular Medicine
Publication Date
2018-07-30
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Cancer Biology | Cell Biology | Cells | Cellular and Molecular Physiology | Developmental Biology | Genetic Phenomena | Molecular Biology
Abstract
Cancer cachexia (CC) is a multifactorial syndrome with an unknown etiology. The primary symptom is the progressive reduction of the body weight. Recently, down-regulation of adipogenic and lipogenic genes were demonstrated to be early affected during cachexia progression in adipose tissue (AT), resulting in AT remodeling. Thus, this study aimed to evaluate in a co-culture system the influence of the Lewis Lung Carcinoma (LLC) tumor cells (c/c-LLC) in an established pre-adipocyte cell line 3T3-L1 adipogenic capacity. c/c-LLC in the presence of 3T3-L1 caused a reduction in lipids accumulation, suggesting that secretory tumor cells products may affect adipogenesis. Interestingly, a very early (day 2) down-regulation of proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha (C/EBPalpha), followed by late genes (day 4 and 8), adiponectin, perilipin, and fatty acid-binding protein 4 (FABP4). Caspase-3 expression was increased on the last day of cell differentiation; it occurred in the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha). Overall, our results suggest that LLC secretory products impair adipocyte differentiation in a co-culture system and increased apoptosis. In summary, our study has shown the inhibition of the adipogenic process in the 3T3-L1 co-culture system with LLC cells.
Keywords
Cancer research, Cell biology, Molecular biology
Rights and Permissions
Copyright 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
DOI of Published Version
10.1016/j.heliyon.2018.e00708
Source
Heliyon. 2018 Jul 30;4(7):e00708. doi: 10.1016/j.heliyon.2018.e00708. eCollection 2018 Jul. Link to article on publisher's site
Journal/Book/Conference Title
Heliyon
Related Resources
PubMed ID
30094378
Repository Citation
Lopes, Magno Alves; Oliveira Franco, Felipe; Henriques, Felipe; Peres, Sidney Barnabe; and Batista, Miguel Luiz Jr., "LLC tumor cells-derivated factors reduces adipogenesis in co-culture system" (2018). Open Access Articles. 3544.
https://escholarship.umassmed.edu/oapubs/3544
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Cancer Biology Commons, Cell Biology Commons, Cells Commons, Cellular and Molecular Physiology Commons, Developmental Biology Commons, Genetic Phenomena Commons, Molecular Biology Commons