Program in Bioinformatics and Integrative Biology
Amino Acids, Peptides, and Proteins | Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Cell Biology | Cells | Developmental Biology | Embryonic Structures | Genetic Phenomena | Genetics and Genomics
The linker histone H1 is critical to maintenance of higher-order chromatin structures and to gene expression regulation. However, H1 dynamics and its functions in embryonic development remain unresolved. Here, we profiled gene expression, nucleosome positions, and H1 locations in early Drosophila embryos. The results show that H1 binding is positively correlated with the stability of beads-on-a-string nucleosome organization likely through stabilizing nucleosome positioning and maintaining nucleosome spacing. Strikingly, nucleosomes with H1 placement deviating to the left or the right relative to the dyad shift to the left or the right, respectively, during early Drosophila embryonic development. H1 occupancy on genic nucleosomes is inversely correlated with nucleosome distance to the transcription start sites. This inverse correlation reduces as gene transcription levels decrease. Additionally, H1 occupancy is lower at the 5' border of genic nucleosomes than that at the 3' border. This asymmetrical pattern of H1 occupancy on genic nucleosomes diminishes as gene transcription levels decrease. These findings shed new lights into how H1 placement dynamics correlates with nucleosome positioning and gene transcription during early Drosophila embryonic development.
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DOI of Published Version
Cell Death Dis. 2018 Jul 9;9(7):765. doi: 10.1038/s41419-018-0819-z. Link to article on publisher's site
Cell death and disease
Hu J, Gu L, Ye Y, Zheng M, Xu Z, Lin J, Du Y, Tian M, Luo L, Wang B, Zhang X, Weng Z, Jiang C. (2018). Dynamic placement of the linker histone H1 associated with nucleosome arrangement and gene transcription in early Drosophila embryonic development. Open Access Articles. https://doi.org/10.1038/s41419-018-0819-z. Retrieved from https://escholarship.umassmed.edu/oapubs/3538
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Amino Acids, Peptides, and Proteins Commons, Biochemistry, Biophysics, and Structural Biology Commons, Bioinformatics Commons, Cell Biology Commons, Cells Commons, Developmental Biology Commons, Embryonic Structures Commons, Genetic Phenomena Commons, Genetics and Genomics Commons