UMMS Affiliation

Department of Microbiology and Physiological Systems; Center for Microbiome Research

Publication Date

5-30-2018

Document Type

Article

Disciplines

Dietetics and Clinical Nutrition | Food Microbiology | Microbial Physiology | Nutrition

Abstract

Human milk contains a high concentration of indigestible oligosaccharides, which likely mediated the coevolution of the nursing infant with its gut microbiome. Specifically, Bifidobacterium longum subsp. infantis (B. infantis) often colonizes the infant gut and utilizes these human milk oligosaccharides (HMOs) to enrich their abundance. In this study, the physiology and mechanisms underlying B. infantis utilization of two HMO isomers lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT) was investigated in addition to their carbohydrate constituents. Both LNT and LNnT utilization induced a significant shift in the ratio of secreted acetate to lactate (1.7-2.0) in contrast to the catabolism of their component carbohydrates (~1.5). Inefficient metabolism of LNnT prompts B. infantis to shunt carbon toward formic acid and ethanol secretion. The global transcriptome presents genomic features differentially expressed to catabolize these two HMO species that vary by a single glycosidic linkage. Furthermore, a measure of strain-level variation exists between B. infantis isolates. Regardless of strain, inefficient HMO metabolism induces the metabolic shift toward formic acid and ethanol production. Furthermore, bifidobacterial metabolites reduced LPS-induced inflammation in a cell culture model. Thus, differential metabolism of milk glycans potentially drives the emergent physiology of host-microbial interactions to impact infant health.

Keywords

Bifidobacteria, host-microbial interactions, human milk oligosaccharide, lacto-N-neotetraose. lacto-N-tetraose, microbiota

Rights and Permissions

Copyright © 2018 Ozcan and Sela. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

DOI of Published Version

10.3389/fnut.2018.00046

Source

Front Nutr. 2018 May 30;5:46. doi: 10.3389/fnut.2018.00046. eCollection 2018. Link to article on publisher's site

Journal/Book/Conference Title

Frontiers in nutrition

Related Resources

Link to Article in PubMed

PubMed ID

29900174

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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