UMMS Affiliation
Department of Neurology
Publication Date
2018-06-01
Document Type
Article
Disciplines
Developmental Neuroscience | Molecular and Cellular Neuroscience | Nervous System Diseases
Abstract
EphrinB3 is important in the regulation of cell proliferation, differentiation and migration via cellcell contact, and can activate the reelin pathway during brain development. However, the effect of ephrinB3 on hippocampal neurogenesis and the reelin pathway in epilepsy remains to be fully elucidated. In the present study, the expression of ephrinB3 in pilocarpineinduced status epilepticus (SE) rats was investigated. SYBR Greenbased reverse transcriptionquantitative polymerase chain reaction analysis, immunohistochemical labeling and western blot analysis were used to detect the gene and protein expression levels of ephrinB3 and reelin pathway proteins. Immunofluorescence staining of doublecortin (DCX) was utilized to analyze hippocampal neurogenesis. The data revealed that the mRNA and protein expression levels of ephrinB3 in the hippocampus decreased during the spontaneous seizure period. Of note, the expression of reelin and its downstream phosphorylation disabled 1 (pDab1) were also notably decreased during the spontaneous seizure period, which showed similar dynamic changes as in the expression of ephrinB3. In addition, it was found that the number of DCXlabeled neuronal progenitor cells was increased in the hippocampus following pilocarpineinduced SE. To further clarify the role of ephrinB3 in neurogenesis and the reelin pathway in epilepsy, an exogenous ephrinB3 clustering stimulator, EphB3Fc, was infused into the bilateral hippocampus of the rats postSE. Following EphB3Fc injection, it was found that the expression levels of reelin and pDab1 were significantly increased in the epileptic rats following EphB3Fc injection. The number of DCXlabeled neuronal progenitor cells was reduced in the hippocampus of the epileptic rats. Furthermore, the intensity and frequency of spontaneous recurrent seizures and electroencephalographic seizures were attenuated in the epileptic rats postinjection. These results demonstrated the critical role of ephrinB3 in regulation of the reelin pathway and hippocampal neurogenesis in epilepsy, providing experimental evidence that ephrinB3 functions as a potential protective factor in epilepsy, at least in animals.
Keywords
epilepsy, ephrin‑B3, reelin pathway, neurogenesis, neuronal excitability
Rights and Permissions
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.
DOI of Published Version
10.3892/ijmm.2018.3543
Source
Int J Mol Med. 2018 Jun;41(6):3457-3467. doi: 10.3892/ijmm.2018.3543. Epub 2018 Mar 7. Link to article on publisher's site
Journal/Book/Conference Title
International journal of molecular medicine
Related Resources
PubMed ID
29512697
Repository Citation
Liu, Tian-Tian; Li, Yi; Shu, Yi; Xiao, Bo; and Feng, Li, "Ephrinb3 modulates hippocampal neurogenesis and the reelin signaling pathway in a pilocarpineinduced model of epilepsy" (2018). Open Access Articles. 3418.
https://escholarship.umassmed.edu/oapubs/3418
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Developmental Neuroscience Commons, Molecular and Cellular Neuroscience Commons, Nervous System Diseases Commons