Department of Medicine, Division of General Internal Medicine
Immunology of Infectious Disease | Immunoprophylaxis and Therapy | Microbial Physiology | Virology | Virus Diseases
Hepatitis B surface antibody (HBsAb) plays a critical role in protecting against infection of hepatitis B virus (HBV) and were extensively studied in literature. At the same time, the status of hepatitis B surface antigen (HBs)-specific B cells in both vaccinated and HBV infected people received limited attention. In the current study, we adopted a highly specific B-cell Enzyme Linked ImmunoSpot (ELISpot) assay to analyze HBs-specific B cells in various clinical settings: healthy individuals with the history of HBV vaccination before and after receiving an extra HBV vaccine boost, people chronically infected with HBV (CHB) in various clinical stages, with or without a particular type anti-viral treatment, or whether receiving a dose of HBV vaccine. In all of these cases, B-cell ELISpot assay was used effectively in enumerating the frequency of HBs-specific B cells. While the focus of the current report was to establish the utility of this assay for HBV research, a number of interesting observations were made in this pilot study based on the profiles and dynamics of HBs-specific B cells in various conditions. Such information is useful to guide the future work in designing novel therapeutic strategies against CHB.
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Copyright © The Author(s) 2018. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DOI of Published Version
Emerg Microbes Infect. 2018 Feb 16;7(1):16. doi: 10.1038/s41426-018-0034-0. Link to article on publisher's site
Emerging microbes and infections
Tian, Chen; Chen, Yuxin; Liu, Yong; Wang, Shixia; Li, Yang; Wang, Guiyang; Xia, Juan; Zhao, Xiang-An; Huang, Rui; Lu, Shan; and Wu, Chao, "Use of ELISpot assay to study HBs-specific B cell responses in vaccinated and HBV infected humans" (2018). Open Access Articles. 3394.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.