Department of Molecular, Cell and Cancer Biology
Biochemistry | Immunology of Infectious Disease | Molecular Biology | Virology | Viruses
We recently identified the multipass transmembrane protein SERINC5 as an antiviral protein that can potently inhibit HIV-1 infectivity and is counteracted by HIV-1 Nef. We now report that the anti-HIV-1 activity, but not the sensitivity to Nef, is conserved among vertebrate SERINC5 proteins. However, a Nef-resistant SERINC5 became Nef sensitive when its intracellular loop 4 (ICL4) was replaced by that of Nef-sensitive human SERINC5. Conversely, human SERINC5 became resistant to Nef when its ICL4 was replaced by that of a Nef-resistant SERINC5. In general, ICL4 regions from SERINCs that exhibited resistance to a given Nef conferred resistance to the same Nef when transferred to a sensitive SERINC, and vice versa. Our results establish that human SERINC5 can be modified to restrict HIV-1 infectivity even in the presence of Nef.
HIV-1, Nef, SERINC5, restriction factor
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Copyright 2017 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI of Published Version
Cell Rep. 2018 Jan 23;22(4):869-875. doi: 10.1016/j.celrep.2017.12.082. Epub 2018 Jan 28. Link to article on publisher's site
Dai W, Usami Y, Wu Y, Gottlinger HG. (2018). A Long Cytoplasmic Loop Governs the Sensitivity of the Anti-viral Host Protein SERINC5 to HIV-1 Nef. Open Access Articles. https://doi.org/10.1016/j.celrep.2017.12.082. Retrieved from https://escholarship.umassmed.edu/oapubs/3363
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