Department of Neurobiology
Neuroscience and Neurobiology
Quantitative assessments of neuronal subtypes in numerous brain regions show large variations in dendritic arbor size. A critical experimental factor is the method used to visualize neurons. We chose to investigate quantitative differences in basolateral amygdala (BLA) principal neuron morphology using two of the most common visualization methods: Golgi-Cox staining and neurobiotin (NB) filling. We show in 8-week-old Wistar rats that NB-filling reveals significantly larger dendritic arbors and different spine densities, compared to Golgi-Cox-stained BLA neurons. Our results demonstrate important differences and provide methodological insights into quantitative disparities of BLA principal neuron morphology reported in the literature.
Golgi–Cox, basolateral amygdala, dendrites, neurobiotin, principal neuron, spines
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© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI of Published Version
Brain Sci. 2017 Dec 19;7(12). pii: brainsci7120165. doi: 10.3390/brainsci7120165. Link to article on publisher's site
Klenowski PM, Wright SE, Mu EW, Noakes PG, Lavidis NA, Bartlett SE, Bellingham MC, Fogarty MJ. (2017). Investigating Methodological Differences in the Assessment of Dendritic Morphology of Basolateral Amygdala Principal Neurons-A Comparison of Golgi-Cox and Neurobiotin Electroporation Techniques. Open Access Articles. https://doi.org/10.3390/brainsci7120165. Retrieved from https://escholarship.umassmed.edu/oapubs/3324
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