Drosophila Model of Leishmania amazonensis Infection
Division of Infectious Diseases and Immunology, Department of Medicine
Immunology and Infectious Disease | Research Methods in Life Sciences
This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.
Amastigote, Axenic amastigote, Drosophila melanogaster, Hemocytes, Leishmania, Phagocytosis, Plasmatocytes, RNAi screen
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Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.
DOI of Published Version
Bio Protoc. 2017 Dec 5;7(23). doi: 10.21769/BioProtoc.2640. Link to article on publisher's site
Okuda K, Silverman NS. (2017). Drosophila Model of Leishmania amazonensis Infection. Open Access Publications by UMMS Authors. https://doi.org/10.21769/BioProtoc.2640. Retrieved from https://escholarship.umassmed.edu/oapubs/3321