Title

Drosophila Model of Leishmania amazonensis Infection

UMMS Affiliation

Division of Infectious Diseases and Immunology, Department of Medicine

Publication Date

12-21-2017

Document Type

Article

Disciplines

Immunology and Infectious Disease | Research Methods in Life Sciences

Abstract

This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.

Keywords

Amastigote, Axenic amastigote, Drosophila melanogaster, Hemocytes, Leishmania, Phagocytosis, Plasmatocytes, RNAi screen

Rights and Permissions

Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

DOI of Published Version

10.21769/BioProtoc.2640

Source

Bio Protoc. 2017 Dec 5;7(23). doi: 10.21769/BioProtoc.2640. Link to article on publisher's site

Journal/Book/Conference Title

Bio-protocol

Related Resources

Link to Article in PubMed

PubMed ID

29276726

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