Infection of Caenorhabditis elegans with Vesicular Stomatitis Virus via Microinjection
RNA Therapeutics Institute
Bacterial Infections and Mycoses | Immunology and Infectious Disease | Investigative Techniques | Pathogenic Microbiology | Research Methods in Life Sciences | Viruses
Over the past 15 years, the free-living nematode, Caenorhabditis elegans has become an important model system for exploring eukaryotic innate immunity to bacterial and fungal pathogens. More recently, infection models using either natural or non-natural nematode viruses have also been established in C. elegans. These models offer new opportunities to use the nematode to understand eukaryotic antiviral defense mechanisms. Here we report protocols for the infection of C. elegans with a non-natural viral pathogen, vesicular stomatitis virus (VSV) through microinjection. We also describe how recombinant VSV strains encoding fluorescent or luciferase reporter genes can be used in conjunction with simple fluorescence-, survival-, and luminescence-based assays to identify host genetic backgrounds with differential susceptibilities to virus infection.
C. elegans, Microinjection, Vesicular stomatitis virus, Virus-host interactions
DOI of Published Version
Bio Protoc. 2017 Nov 20;7(22). doi: 10.21769/BioProtoc.2617. Link to article on publisher's site
Martin, Adam; Rex, Emily A.; Ishidate, Takao; Lin, Rueyling; and Gammon, Don B., "Infection of Caenorhabditis elegans with Vesicular Stomatitis Virus via Microinjection" (2017). Open Access Articles. 3305.