Title

Infection of Caenorhabditis elegans with Vesicular Stomatitis Virus via Microinjection

UMMS Affiliation

RNA Therapeutics Institute

Publication Date

2017-11-20

Document Type

Article

Disciplines

Bacterial Infections and Mycoses | Immunology and Infectious Disease | Investigative Techniques | Pathogenic Microbiology | Research Methods in Life Sciences | Viruses

Abstract

Over the past 15 years, the free-living nematode, Caenorhabditis elegans has become an important model system for exploring eukaryotic innate immunity to bacterial and fungal pathogens. More recently, infection models using either natural or non-natural nematode viruses have also been established in C. elegans. These models offer new opportunities to use the nematode to understand eukaryotic antiviral defense mechanisms. Here we report protocols for the infection of C. elegans with a non-natural viral pathogen, vesicular stomatitis virus (VSV) through microinjection. We also describe how recombinant VSV strains encoding fluorescent or luciferase reporter genes can be used in conjunction with simple fluorescence-, survival-, and luminescence-based assays to identify host genetic backgrounds with differential susceptibilities to virus infection.

Keywords

C. elegans, Microinjection, Vesicular stomatitis virus, Virus-host interactions

DOI of Published Version

10.21769/BioProtoc.2617

Source

Bio Protoc. 2017 Nov 20;7(22). doi: 10.21769/BioProtoc.2617. Link to article on publisher's site

Journal/Book/Conference Title

Bio-protocol

Related Resources

Link to Article in PubMed

PubMed ID

29276724

Share

COinS