Department of Microbiology and Physiological Systems; Department of Pathology
Digestive System | Digestive System Diseases | Immunology and Infectious Disease | Medical Immunology
Understanding the transcription factors that modulate epithelial resistance to injury is necessary for understanding intestinal homeostasis and injury repair processes. Recently, transcription factor EB (TFEB) was implicated in expression of autophagy and host defense genes in nematodes and mammalian cells. However, the in vivo roles of TFEB in the mammalian intestinal epithelium were not known. Here, we used mice with a conditional deletion of Tfeb in the intestinal epithelium (Tfeb (DeltaIEC)) to examine its importance in defense against injury. Unperturbed Tfeb (DeltaIEC) mice exhibited grossly normal intestinal epithelia, except for a defect in Paneth cell granules. Tfeb (DeltaIEC) mice exhibited lower levels of lipoprotein ApoA1 expression, which is downregulated in Crohn's disease patients and causally linked to colitis susceptibility. Upon environmental epithelial injury using dextran sodium sulfate (DSS), Tfeb (DeltaIEC) mice exhibited exaggerated colitis. Thus, our study reveals that TFEB is critical for resistance to intestinal epithelial cell injury, potentially mediated by APOA1.
Gastrointestinal models, Mucosal immunology
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DOI of Published Version
Sci Rep. 2017 Oct 24;7(1):13938. doi: 10.1038/s41598-017-14370-4. Link to article on publisher's site
Murano T, Najibi M, Paulus GL, Adiliaghdam F, Valencia-Guerrero A, Selig M, Wang X, Jeffrey K, Xavier RJ, Lassen KG, Irazoqui JE. (2017). Transcription factor TFEB cell-autonomously modulates susceptibility to intestinal epithelial cell injury in vivo. Open Access Articles. https://doi.org/10.1038/s41598-017-14370-4. Retrieved from https://escholarship.umassmed.edu/oapubs/3290
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This work is licensed under a Creative Commons Attribution 4.0 License.