UMMS Affiliation

Department of Molecular, Cell, and Cancer Biology

Publication Date


Document Type



Animal Experimentation and Research | Genetics and Genomics | Nucleic Acids, Nucleotides, and Nucleosides


The zebrafish (Danio rerio) has emerged as a powerful model to study vertebrate development and disease. Its short generation time makes it amenable to genetic manipulation and analysis, and its small size and high fecundity make it especially well suited for large-scale forward genetic and chemical screens. Fast-developing zebrafish embryos are transparent, facilitating live imaging of a variety of developmental processes in wild-type and mutant animals. ...

This brief document provides an updated set of guidelines regarding morpholino use in zebrafish that we anticipate will be of value for experimentalists as well as journal and grant reviewers, and decision makers.


morpholinos, zebrafish, morpholino antisense oligomers, gene knockdown

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Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

DOI of Published Version



PLoS Genet. 2017 Oct 19;13(10):e1007000. doi: 10.1371/journal.pgen.1007000. eCollection 2017 Oct. Link to article on publisher's site

Journal/Book/Conference Title

PLoS genetics


Full author list omitted for brevity. For the full list of authors, see article.

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PubMed ID


Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons 1.0 Public Domain Dedication.