Title

Systemic gene delivery transduces the enteric nervous system of guinea pigs and cynomolgus macaques

UMMS Affiliation

Department of Pediatrics, Division of Pediatric Pulmonology; Gene Therapy Center

Publication Date

2017-10-01

Document Type

Article

Disciplines

Animal Experimentation and Research | Genetic Phenomena | Genetics and Genomics | Molecular and Cellular Neuroscience | Nervous System | Therapeutics

Abstract

Characterization of adeno-associated viral vector (AAV) mediated gene delivery to the enteric nervous system (ENS) was recently described in mice and rats. In these proof-of-concept experiments, we show that intravenous injections of clinically relevant AAVs can transduce the ENS in guinea pigs and non-human primates. Neonatal guinea pigs were given intravenous injections of either AAV8 or AAV9 vectors that contained a green fluorescent protein (GFP) expression cassette or phosphate-buffered saline. Piglets were euthanized three weeks post injection and tissues were harvested for immunofluorescent analysis. GFP expression was detected in myenteric and submucosal neurons along the length of the gastrointestinal tract in AAV8 injected guinea pigs. GFP-positive neurons were found in dorsal motor nucleus of the vagus and dorsal root ganglia. Less transduction occurred in AAV9-treated tissues. Gastrointestinal tissues were analyzed from young cynomolgus macaques that received systemic injection of AAV9 GFP. GFP expression was detected in myenteric neurons of the stomach, small and large intestine. These data demonstrate that ENS gene delivery translates to larger species. This work develops tools for the field of neurogastroenterology to explore gut physiology and anatomy using emerging technologies such as optogenetics and gene editing. It also provides a basis to develop novel therapies for chronic gut disorders.

Keywords

Cellular neuroscience, Peripheral nervous system

DOI of Published Version

10.1038/gt.2017.72

Source

Gene Ther. 2017 Oct;24(10):640-648. doi: 10.1038/gt.2017.72. Epub 2017 Aug 3. Link to article on publisher's site

Journal/Book/Conference Title

Gene therapy

Related Resources

Link to Article in PubMed

PubMed ID

28771235

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