Diabetes Center of Excellence; Program in Molecular Medicine
Cell Biology | Immunity | Molecular, Cellular, and Tissue Engineering | Therapeutics | Tissues
There is growing interest in using embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) derivatives for tissue regeneration. However, an increased understanding of human immune responses to stem cell-derived allografts is necessary for maintaining long-term graft persistence. To model this alloimmunity, humanized mice engrafted with human hematopoietic and immune cells could prove to be useful. In this study, an in-depth analysis of graft-infiltrating human lymphocytes and splenocytes revealed that humanized mice incompletely model human immune responses toward allogeneic stem cells and their derivatives. Furthermore, using an "allogenized" mouse model, we show the feasibility of reconstituting immunodeficient mice with a functional mouse immune system and describe a key role of innate immune cells in the rejection of mouse stem cell allografts.
T cell exhaustion, allograft, humanized mice, immunogenicity, pluripotent stem cells, stem cell therapeutics, wasting disease
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© 2017 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
DOI of Published Version
Cell Rep. 2017 Aug 22;20(8):1978-1990. doi: 10.1016/j.celrep.2017.08.003. Link to article on publisher's site
Kooreman, Nigel G.; Brehm, Michael A.; Greiner, Dale L.; Shultz, Leonard D.; and Wu, Joseph C., "Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics" (2017). Open Access Articles. 3224.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.