Adeno-associated virus serotype rh.10 displays strong muscle tropism following intraperitoneal delivery
UMass Chan Affiliations
Department of Microbiology and Physiological SystemsHorae Gene Therapy Center
Document Type
Journal ArticlePublication Date
2017-01-09Keywords
Applied microbiologyGene therapy
Genetic transduction
Translational research
Genetics and Genomics
Therapeutics
Metadata
Show full item recordAbstract
Recombinant adeno-associated virus (rAAV) is an attractive tool for basic science and translational medicine including gene therapy, due to the versatility in its cell and organ transduction. Previous work indicates that rAAV transduction patterns are highly dependent on route of administration. Based on this relationship, we hypothesized that intraperitoneal (IP) administration of rAAV produces unique patterns of tissue tropism. To test this hypothesis, we investigated the transduction efficiency of 12 rAAV serotypes carrying an enhanced green fluorescent protein (EGFP) reporter gene in a panel of 12 organs after IP injection. Our data suggest that IP administration emphasizes transduction patterns that are different from previously reported intravascular delivery methods. Using this approach, rAAV efficiently transduces the liver, pancreas, skeletal muscle, heart and diaphragm without causing significant histopathological changes. Of note, rAAVrh.10 showed excellent muscle transduction following IP administration, highlighting its potential as a new muscle-targeting vector.Source
Sci Rep. 2017 Jan 9;7:40336. doi: 10.1038/srep40336. Link to article on publisher's siteDOI
10.1038/srep40336Permanent Link to this Item
http://hdl.handle.net/20.500.14038/40276PubMed ID
28067312Related Resources
Link to Article in PubMedRights
Copyright © 2017, The Author(s).Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1038/srep40336