UMMS Affiliation
Graduate School of Biomedical Sciences; School of Medicine
Publication Date
2016-11-11
Document Type
Article
Disciplines
Cancer Biology | Cell Biology
Abstract
BACKGROUND: The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitate these genetic analyses.
RESULTS: We report here a simple, one-step immortalization method that involves retroviral vector mediated co-expression of the human telomerase protein and a shRNA targeting the CDKN2A gene locus. We demonstrate that this method could successfully immortalize human small airway epithelial cells while maintaining their chromosomal stability. We further showed that these cells retain p53 activity and can be transformed by the KRAS oncogene.
CONCLUSIONS: Our method simplifies the immortalization process and is broadly applicable for establishing immortalized epithelial cell lines from primary human tissues for cancer research.
Rights and Permissions
© The Author(s) 2016.
DOI of Published Version
10.1186/s13578-016-0122-6
Source
Cell Biosci. 2016 Nov 11;6:57. eCollection 2016. Link to article on publisher's site
Journal/Book/Conference Title
Cell and bioscience
Related Resources
PubMed ID
27891214
Repository Citation
Smith, Jordan L.; Lee, Liam C.; Read, Abigail; Li, Qiuning; Yu, Bing; Lee, Chih-Shia; and Luo, Ji, "One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation" (2016). Open Access Articles. 2992.
https://escholarship.umassmed.edu/oapubs/2992
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.