Graduate School of Biomedical Sciences; School of Medicine
Cancer Biology | Cell Biology
BACKGROUND: The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitate these genetic analyses.
RESULTS: We report here a simple, one-step immortalization method that involves retroviral vector mediated co-expression of the human telomerase protein and a shRNA targeting the CDKN2A gene locus. We demonstrate that this method could successfully immortalize human small airway epithelial cells while maintaining their chromosomal stability. We further showed that these cells retain p53 activity and can be transformed by the KRAS oncogene.
CONCLUSIONS: Our method simplifies the immortalization process and is broadly applicable for establishing immortalized epithelial cell lines from primary human tissues for cancer research.
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© The Author(s) 2016.
DOI of Published Version
Cell Biosci. 2016 Nov 11;6:57. eCollection 2016. Link to article on publisher's site
Cell and bioscience
Smith JL, Lee LC, Read A, Li Q, Yu B, Lee C, Luo J. (2016). One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation. Open Access Articles. https://doi.org/10.1186/s13578-016-0122-6. Retrieved from https://escholarship.umassmed.edu/oapubs/2992
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This work is licensed under a Creative Commons Attribution 4.0 License.