UMMS Affiliation
RNA Therapeutics Institute; Program in Molecular Medicine; Proteomics and Mass Spectrometry Facility; Department of Biochemistry and Molecular Pharmacology; Department of Medicine
Publication Date
11-17-2016
Document Type
Article
Disciplines
Biochemistry | Cancer Biology | Cell Biology | Genomics
Abstract
Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs). We identified 3,532 proteins and 1,961 lipid species in the screen. Exosomes differed from MVs in several different areas: (a) The protein patterns of exosomes were more likely different from their cells of origin than were the protein patterns of MVs; (b) The proteomes of U87 and Huh7 exosomes were similar to each other but different from the proteomes of MSC exosomes, whereas the lipidomes of Huh7 and MSC exosomes were similar to each other but different from the lipidomes of U87 exosomes; (c) exosomes exhibited proteins of extracellular matrix, heparin-binding, receptors, immune response and cell adhesion functions, whereas MVs were enriched in endoplasmic reticulum, proteasome and mitochondrial proteins. Exosomes and MVs also differed in their types of lipid contents. Enrichment in glycolipids and free fatty acids characterized exosomes, whereas enrichment in ceramides and sphingomyelins characterized MVs. Furthermore, Huh7 and MSC exosomes were specifically enriched in cardiolipins; U87 exosomes were enriched in sphingomyelins. This study comprehensively analyses the protein and lipid composition of exosomes, MVs and source cells in 3 different cell types.
Keywords
cancer, extracellular vesicles, lipidomics, proteomics, stem cells
Rights and Permissions
© 2016 Reka A. Haraszti et al.
DOI of Published Version
10.3402/jev.v5.32570
Source
J Extracell Vesicles. 2016 Nov 17;5:32570. doi: 10.3402/jev.v5.32570. eCollection 2016. Link to article on publisher's website
Journal/Book/Conference Title
Journal of extracellular vesicles
Related Resources
PubMed ID
27863537
Repository Citation
Haraszti, Reka A.; Didiot, Marie-Cecile; Sapp, Ellen; Leszyk, John D.; Shaffer, Scott A.; Rockwell, Hannah E.; Gao, Fei; Narain, Niven R.; DiFiglia, Marian; Kiebish, Michael A.; Aronin, Neil; and Khvorova, Anastasia, "High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources" (2016). Open Access Articles. 2988.
https://escholarship.umassmed.edu/oapubs/2988
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License