UMMS Affiliation
Department of Molecular, Cell and Cancer Biology
Publication Date
11-17-2016
Document Type
Article
Disciplines
Developmental Biology | Developmental Neuroscience
Abstract
Vascular networks surrounding individual organs are important for their development, maintenance, and function; however, how these networks are assembled remains poorly understood. Here we show that CNS progenitors, referred to as radial glia, modulate vascular patterning around the spinal cord by acting as negative regulators. We found that radial glia ablation in zebrafish embryos leads to excessive sprouting of the trunk vessels around the spinal cord, and exclusively those of venous identity. Mechanistically, we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae, and sFlt1 overexpression rescues it. Genetic mosaic analyses show that sFlt1 function in trunk endothelial cells can limit their over-sprouting. Together, our findings identify CNS-resident progenitors as critical angiogenic regulators that determine the precise patterning of the vasculature around the spinal cord, providing novel insights into vascular network formation around developing organs.
Keywords
angiogenic regulation, developmental biology, neuroscience, radial glia, spinal cord, stem cells, vascular patterning, zebrafish
Rights and Permissions
Copyright © 2016, Matsuoka et al.
DOI of Published Version
10.7554/eLife.20253
Source
Elife. 2016 Nov 17;5. pii: e20253. doi: 10.7554/eLife.20253. Link to article on publisher's site
Journal/Book/Conference Title
eLife
Related Resources
PubMed ID
27852438
Repository Citation
Matsuoka, Ryota L.; Marass, Michele; Avdesh, Avdesh; Helker, Christian S.M.; Maischein, Hans-Martin; Grosse, Ann S.; Kaur, Harmandeep; Lawson, Nathan D.; Herzog, Wiebke; and Stainier, Didier Y.R., "Radial glia regulate vascular patterning around the developing spinal cord" (2016). Open Access Articles. 2987.
https://escholarship.umassmed.edu/oapubs/2987
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.