UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Publication Date
2016-04-27
Document Type
Article
Disciplines
Cancer Biology | Cell Biology
Abstract
Increasing evidence shows that long noncoding RNAs (lncRNAs) have important roles in the regulation of multiple cellular processes, including cell division, cell growth, and apoptosis, as well as cancer metastasis and neurological disease progression; however, the mechanism of how lncRNAs regulate these processes is not well established. In this study, we demonstrated that downregulating the expression of the lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) in breast cancer cells inhibited cell growth and induced cell apoptosis. In addition, the RNA-binding protein fused in sarcoma/translocated in liposarcoma (FUS/TLS) physically interacted with NEAT1, and reducing the expression of FUS/TLS also induced cell apoptosis. Multiple miRNAs were identified as regulators of NEAT1, but only overexpression of miR-548ar was able to decrease NEAT1 expression and promote apoptosis. These results indicate a novel interaction between NEAT1, miR-548ar-3p, and FUS and their role in the regulation of breast cancer cell apoptosis.
Keywords
FUS, NEAT1, cell apoptosis, lncRNA, miR-548ar-3p
Rights and Permissions
Copyright the authors, publisher and licensee Libertas Academica Limited.
DOI of Published Version
10.4137/GRSB.S29414
Source
Gene Regul Syst Bio. 2016 Apr 27;10(Suppl 1):11-7. doi: 10.4137/GRSB.S29414. eCollection 2016. Link to article on publisher's site
Journal/Book/Conference Title
Gene regulation and systems biology
Related Resources
PubMed ID
27147820
Repository Citation
Ke H, Zhao L, Feng X, Xu H, Zou L, Yang Q, Su X, Peng L, Jiao B. (2016). NEAT1 is Required for Survival of Breast Cancer Cells Through FUS and miR-548. Open Access Publications by UMass Chan Authors. https://doi.org/10.4137/GRSB.S29414. Retrieved from https://escholarship.umassmed.edu/oapubs/2900
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License