UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute

Publication Date

2016-04-27

Document Type

Article

Disciplines

Cancer Biology | Cell Biology

Abstract

Increasing evidence shows that long noncoding RNAs (lncRNAs) have important roles in the regulation of multiple cellular processes, including cell division, cell growth, and apoptosis, as well as cancer metastasis and neurological disease progression; however, the mechanism of how lncRNAs regulate these processes is not well established. In this study, we demonstrated that downregulating the expression of the lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) in breast cancer cells inhibited cell growth and induced cell apoptosis. In addition, the RNA-binding protein fused in sarcoma/translocated in liposarcoma (FUS/TLS) physically interacted with NEAT1, and reducing the expression of FUS/TLS also induced cell apoptosis. Multiple miRNAs were identified as regulators of NEAT1, but only overexpression of miR-548ar was able to decrease NEAT1 expression and promote apoptosis. These results indicate a novel interaction between NEAT1, miR-548ar-3p, and FUS and their role in the regulation of breast cancer cell apoptosis.

Keywords

FUS, NEAT1, cell apoptosis, lncRNA, miR-548ar-3p

Rights and Permissions

Copyright the authors, publisher and licensee Libertas Academica Limited.

DOI of Published Version

10.4137/GRSB.S29414

Source

Gene Regul Syst Bio. 2016 Apr 27;10(Suppl 1):11-7. doi: 10.4137/GRSB.S29414. eCollection 2016. Link to article on publisher's site

Journal/Book/Conference Title

Gene regulation and systems biology

Related Resources

Link to Article in PubMed

PubMed ID

27147820

Creative Commons License

Creative Commons Attribution-Noncommercial 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License

Download

Share

COinS