Department of Biochemistry and Molecular Pharmacology; Graduate School of Biomedical Sciences, Program in Biochemistry and Molecular Pharmacology
Biochemistry | Cell Biology | Molecular Biology
Trafficking in eukaryotic cells is a tightly regulated process to ensure correct cargo delivery to the proper destination organelle or plasma membrane. In this review, we focus on how the vesicle fusion machinery, the SNARE complex, is regulated by the interplay of the multisubunit tethering complexes (MTC) with the SNAREs and Sec1/Munc18 (SM) proteins. Although these factors are used in different stages of membrane trafficking, e.g., Golgi to plasma membrane transport vs. vacuolar fusion, and in a variety of diverse eukaryotic cell types, many commonalities between their functions are being revealed. We explore the various protein-protein interactions and findings from functional reconstitution studies in order to highlight both their common features and the differences in their modes of regulation. These studies serve as a starting point for mechanistic explorations in other systems.
SNARE complexes, Sec1/Munc18, intracellular trafficking, multisubunit tethering complexes
DOI of Published Version
Front Cell Dev Biol. 2016 May 9;4:42. doi: 10.3389/fcell.2016.00042. eCollection 2016. Link to article on publisher's site
Frontiers in cell and developmental biology
Dubuke ML, Munson M. (2016). The Secret Life of Tethers: The Role of Tethering Factors in SNARE Complex Regulation. Open Access Articles. https://doi.org/10.3389/fcell.2016.00042. Retrieved from https://escholarship.umassmed.edu/oapubs/2897
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This work is licensed under a Creative Commons Attribution 4.0 License.