Department of Microbiology and Physiological Systems
Cell Biology | Cellular and Molecular Physiology
The apical brush border membrane (BBM) of intestinal epithelial cells forms a highly structured and dynamic environmental interface that serves to regulate cellular physiology and block invasion by intestinal microbes and their products. How the BBM dynamically responds to pathogenic and commensal bacterial signals can define intestinal homeostasis and immune function. We previously found that in model intestinal epithelium, the conversion of apical membrane sphingomyelin to ceramide by exogenous bacterial sphingomyelinase (SMase) protected against the endocytosis and toxicity of cholera toxin. Here we elucidate a mechanism of action by showing that SMase induces a dramatic, reversible, RhoA-dependent alteration of the apical cortical F-actin network. Accumulation of apical membrane ceramide is necessary and sufficient to induce the actin phenotype, and this coincides with altered membrane structure and augmented innate immune function as evidenced by resistance to invasion by Salmonella.
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© 2016 Saslowsky, Thiagarajah, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
DOI of Published Version
Mol Biol Cell. 2016 Apr 1;27(7):1120-30. doi: 10.1091/mbc.E15-05-0293. Epub 2016 Feb 10. Link to article on publisher's site
Molecular biology of the cell
Saslowsky DE, Thiagarajah JR, McCormick BA, Lee JC, Lencer WI. (2016). Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion. Open Access Articles. https://doi.org/10.1091/mbc.E15-05-0293. Retrieved from https://escholarship.umassmed.edu/oapubs/2873
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.