UMMS Affiliation
Department of Medicine, Division of Cardiovascular Medicine; Department of Quantitative Health Sciences; UMass Metabolic Network
Publication Date
2016-04-26
Document Type
Article
Disciplines
Computational Biology | Genetics | Genomics | Hemic and Immune Systems
Abstract
There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population.
Keywords
Genetics, Small RNAs
Rights and Permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
DOI of Published Version
10.1038/ncomms11106
Source
Nat Commun. 2016 Apr 26;7:11106. doi: 10.1038/ncomms11106. Link to article on publisher's site
Journal/Book/Conference Title
Nature communications
Related Resources
PubMed ID
27112789
Repository Citation
Freedman, Jane E.; Mick, Eric; Beaulieu, Lea M.; Tanriverdi, Selim E.; Koupenova-Zamor, Milka; Mikhalev, Ekaterina; and Tanriverdi, Kahraman, "Diverse human extracellular RNAs are widely detected in human plasma" (2016). Open Access Articles. 2853.
https://escholarship.umassmed.edu/oapubs/2853
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Computational Biology Commons, Genetics Commons, Genomics Commons, Hemic and Immune Systems Commons
Comments
Full author list omitted for brevity. For full list of authors see article.