Hepatocyte growth factor, hepatocyte growth factor activator and arginine in a rat fulminant colitis model
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Authors
Zwintscher, Nathan P.Shah, Puja M.
Salgar, Shashikumar K.
Newton, Christopher R.
Maykel, Justin A.
Samy, Ahmed
Jabir, Murad
Steele, Scott R.
UMass Chan Affiliations
Department of SurgeryDocument Type
Journal ArticlePublication Date
2016-05-05Keywords
Fulminant colitisInflammatory bowel disease
Digestive System Diseases
Gastroenterology
Surgery
Metadata
Show full item recordAbstract
INTRODUCTION: Dextran sodium sulfate (DSS) is commonly used to induce a murine fulminant colitis model. Hepatocyte growth factor (HGF) has been shown to decrease the symptoms of inflammatory bowel disease (IBD) but the effect of its activator, HGFA, is not well characterized. Arginine reduces effects of oxidative stress but its effect on IBD is not well known. The primary aim is to determine whether HGF and HGFA, or arginine will decrease IBD symptoms such as pain and diarrhea in a DSS-induced fulminant colitis murine model. METHODS: A severe colitis was induced in young, male Fischer 344 rats with 4% (w/v) DSS oral solution for seven days; rats were sacrificed on day 10. Rats were divided into five groups of 8 animals: control, HGF (700 mcg/kg/dose), HGF and HGFA (10 mcg/dose), HGF and arginine, and high dose HGF (2800 mcg/kg/dose). Main clinical outcomes were pain, diarrhea and weight loss. Blinded pathologists scored the terminal ileum and distal colon. RESULTS: DSS reliably induced severe active colitis in 90% of animals (n = 36/40). There were no differences in injury scores between control and treatment animals. HGF led to 1.38 fewer days in pain (p = 0.036), while arginine led to 1.88 fewer days of diarrhea (P = 0.017) compared to controls. 88% of HGFA-treated rats started regaining weight (P < 0.001). DISCUSSION/CONCLUSION: Although treatment was unable to reverse fulminant disease, HGF and arginine were associated with decreased days of pain and diarrhea. These clinical interventions may reduce associated symptoms for severe IBD patients, even when urgent surgical intervention remains the only viable option.Source
Ann Med Surg (Lond). 2016 Apr 5;7:97-103. doi: 10.1016/j.amsu.2016.03.039. eCollection 2016. Link to article on publisher's site
DOI
10.1016/j.amsu.2016.03.039Permanent Link to this Item
http://hdl.handle.net/20.500.14038/40038PubMed ID
27144006Related Resources
Rights
Copyright © 2016 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.amsu.2016.03.039
Scopus Count
Collections
Except where otherwise noted, this item's license is described as Copyright © 2016 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).