Department of Molecular, Cell and Cancer Biology
Cell Biology | Genetics and Genomics
During mitosis chromosomes are condensed to facilitate their segregation, through a process mediated by the condensin complex. Although several factors that promote maximal condensin activity during mitosis have been identified, the mechanisms that downregulate condensin activity during interphase are largely unknown. Here, we demonstrate that Ycg1, the Cap-G subunit of budding yeast condensin, is cell cycle-regulated with levels peaking in mitosis and decreasing as cells enter G1 phase. This cyclical expression pattern is established by a combination of cell cycle-regulated transcription and constitutive degradation. Interestingly, overexpression of YCG1 and mutations that stabilize Ycg1 each result in delayed cell-cycle entry and an overall proliferation defect. Overexpression of no other condensin subunit impacts the cell cycle, suggesting that Ycg1 is limiting for condensin complex formation. Consistent with this possibility, we find that levels of intact condensin complex are reduced in G1 phase compared to mitosis, and that increased Ycg1 expression leads to increases in both levels of condensin complex and binding to chromatin in G1. Together, these results demonstrate that Ycg1 levels limit condensin function in interphase cells, and suggest that the association of condensin with chromosomes must be reduced following mitosis to enable efficient progression through the cell cycle.
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Copyright © 2016 Doughty et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
PLoS Genet. 2016 Jul 27;12(7):e1006216. doi: 10.1371/journal.pgen.1006216. eCollection 2016.Link to article on publisher's site
Doughty TW, Arsenault HE, Benanti JA. (2016). Levels of Ycg1 Limit Condensin Function during the Cell Cycle. Open Access Articles. https://doi.org/10.1371/journal.pgen.1006216. Retrieved from https://escholarship.umassmed.edu/oapubs/2804
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This work is licensed under a Creative Commons Attribution 4.0 License.