UMMS Affiliation
RNA Therapeutics Institute; Program in Molecular Medicine
Publication Date
2016-02-18
Document Type
Article
Disciplines
Biotechnology | Computational Biology | Genetics | Systems Biology
Abstract
Traditional cloning methods have limitations on the number of DNA fragments that can be simultaneously manipulated, which dramatically slows the pace of molecular assembly. Here we describe GMAP, a Gibson assembly-based modular assembly platform consisting of a collection of promoters and genes, which allows for one-step production of DNA constructs. GMAP facilitates rapid assembly of expression and viral constructs using modular genetic components, as well as increasingly complicated genetic tools using contextually relevant genomic elements. Our data demonstrate the applicability of GMAP toward the validation of synthetic promoters, identification of potent RNAi constructs, establishment of inducible lentiviral systems, tumor initiation in genetically engineered mouse models, and gene-targeting for the generation of knock-in mice. GMAP represents a recombinant DNA technology designed for widespread circulation and easy adaptation for other uses, such as synthetic biology, genetic screens, and CRISPR-Cas9.
Rights and Permissions
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DOI of Published Version
10.1038/srep16836
Source
Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. Link to article on publisher's site
Journal/Book/Conference Title
Scientific reports
Related Resources
PubMed ID
26887506
Repository Citation
Akama-Garren, Elliot; Joshi, Nik; Tammela, Tuomas; Chang, Gregory P.; Wagner, Bethany L.; Lee, Da-Yae; Rideout Iii, William M.; Papagiannakopoulos, Thales; Xue, Wen; and Jacks, Tyler, "A Modular Assembly Platform for Rapid Generation of DNA Constructs" (2016). Open Access Articles. 2747.
https://escholarship.umassmed.edu/oapubs/2747
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Biotechnology Commons, Computational Biology Commons, Genetics Commons, Systems Biology Commons