Department of Biochemistry and Molecular Pharmacology
Biochemistry | Cell Biology | Genetics
The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed "Opto-CRAC") that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded "photoactivatable adjuvant" to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote control of Ca2+-modulated activities with tailored function.
Calcium signaling, Immune response, Nanoparticles, Near infrared, Optogenetics, STIM1, biochemistry, cell biology, human, mouse
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© 2015, He et al. This article is distributed under the terms of theCreative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI of Published Version
Elife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024. Link to article on publisher's site
He L, Zhang Y, Ma G, Tan P, Li ZJ, Zhang S, Wu X, Jing J, Fang S, Zhou L, Wang Y, Huang Y, Hogan P, Han G, Zhou Y. (2015). Near-infrared photoactivatable control of Ca signaling and optogenetic immunomodulation. Open Access Publications by UMMS Authors. https://doi.org/10.7554/eLife.10024. Retrieved from https://escholarship.umassmed.edu/oapubs/2645
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This work is licensed under a Creative Commons Attribution 4.0 License.